Antibody detailed product information

Code No.	MD-11-3
Anti-Fas (CD95) (Human) mAb
Size	100 µL (1 mg/mL)
Availability (in Japan)	1-9 （In Japan at 00:05, Apr 18, 2024 in JST）
Clonality	Monoclonal
Clone	ZB4
Isotype (Immunized Animal)	Mouse IgG1
Applications	WB　5 µg/mL FCM　5-20 µg/mL (final concentration)
Immunogen (Antigen)	Recombinant Human Fas
Reactivity [Gene ID]	Human[355]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Alternative names	Fas (TNF receptor superfamily, member 6), APT1, CD95, FAS1, APO-1, FASTM, ALPS1A, TNFRSF6
Background	It is now widely accepted that apoptosis plays an important role in the selection of immature thymocytes and Ag-primed peripheral T cells. Fas (also known as CD95/APO-1) is a cell surface protein belonging to the tumor necrosis factor receptor superfamily, which is expressed in a variety of normal and neoplastic cells. Binding of FasL to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies results in rapid induction of apoptosis in Fas expressing cells. Clone ZB4 is known as Fas blocking, Fas neutralizing or Fas antagonistic antibody, whereas anti-Fas antibody (clone CH-11) is known as the agonistic antibody.
Related products	SY-001 Anti-Fas (CD95) (Human) mAb MD-10-3 Anti-Fas (CD95) (Human) mAb MD-10-4 Anti-Fas (CD95) (Human) mAb-FITC MD-10-5 Anti-Fas (CD95) (Human) mAb-PE
Product category	Research area:Apoptosis Cell surface antigens
Citations	Zanin-Zhorov A et al. Fibronectin-associated Fas ligand rapidly induces opposing and time-dependent effects on the activation and apoptosis of T cells. J Immunol. 171, 5882-9 (2003)(PMID:14634098) Western Blotting Liu W et al. Hepatitis B virus core protein inhibits Fas-mediated apoptosis of hepatoma cells via regulation of mFas/FasL and sFas expression. FASEB J. 29, 1113-23 (2015)(PMID:25466893) Neutralization Chiou SH et al. Up-regulation of Fas ligand expression by human cytomegalovirus immediate-early gene product 2: a novel mechanism in cytomegalovirus-induced apoptosis in human retina. J Immunol. 167, 4098-103 (2001)(PMID:11564832) Chiou SH et al. The immediate early 2 protein of human cytomegalovirus (HCMV) mediates the apoptotic control in HCMV retinitis through up-regulation of the cellular FLICE-inhibitory protein expression. J Immunol. 177, 6199-206 (2006)(PMID:17056549) Function Jing ZT et al. Hepatitis B Virus Surface Antigen Enhances the Sensitivity of Hepatocytes to Fas-Mediated Apoptosis via Suppression of AKT Phosphorylation. J Immunol. 201, 2303-2314 (2018)(PMID:30171166)

The availability is based on the information in Japan at 00:05, Apr 18, 2024 in JST.
The special price is shown in red color.
Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.