Antibody detailed product information

Code No.	M084-5
Anti-Podocalyxin (PCLP1) (Human) mAb-PE
Size	1 mL (50 tests)
Availability (in Japan)	10 or more （In Japan at 00:05, Apr 19, 2024 in JST）
Clonality	Monoclonal
Clone	53D11
Isotype (Immunized Animal)	Mouse IgG2a
Applications	FCM　20 µL (Ready for use.)
Immunogen (Antigen)	CHO cell expressing full-length human Podocalyxin/PCLP1
Reactivity [Gene ID]	Human[5420]
Storage buffer	PBS/1% BSA/0.09% NaN₃
Storage temp.	4°C
Conjugate	PE
Manufacturer	MBL
Alternative names	PODXL, Gp200, PC, PCLP,
Background	Recent studies with avian embryos and murine embryonic stem cells have suggested that hematopoietic cells are derived from hemangioblasts, the common precursors of hematopoietic and endothelial cells. Hara et al. molecularly cloned podocalyxin-like protein 1 (PCLP1) as a novel surface marker for endothelial-like cells in the AGM (aorta-gonad-mesonephros) region of mouse embryos, where long-term repopulating hematopoietic stem cells (LTR-HSCs) are known to arise. PCLP1⁺CD45^- cells in the AGM region incorporated acetylated low-density lipoprotein and produced both hematopoietic and endothelial cells when cocultured with OP9 stromal cells. Moreover, multiple lineages of hematopoietic cells were generated in vivo when PCLP1⁺ CD45^- cells were injected into neonatal liver of busulfan-treated mice. Today it is reported that the PCLP1 is identical with the Podocalyxin.
Related products	M084-3 Anti-Podocalyxin (PCLP1) (Human) mAb M084-4 Anti-Podocalyxin (PCLP1) (Human) mAb-FITC
Product category	Research area:Stem cell research Cell surface antigens

The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
The special price is shown in red color.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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