Antibody detailed product information

Code No. K0205-3
Anti-Txnip (VDUP1) mAb
Size 100 µL (1 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 00:05, Apr 20, 2024 in JST）
Clonality Monoclonal
Clone JY2
Isotype
(Immunized Animal) 		Mouse IgG1
Applications WB　1 µg/mL  
IP　2 µg/200 µL of cell extract from 5x106 cells  
IC*　reported.  （PMID: 27929088）
IH*　reported.  （PMID: 19959470 / 23584857）
Immunogen
(Antigen)		 Recombinant Human Txnip/VDUP1
Reactivity [Gene ID]

Human[10628], Mouse[56338], Rat*[117514](PMID: 19258488/23803610/26858253)
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names thioredoxin interacting protein, THIF, VDUP1
Background Thioredoxin (TRX)-binding protein (Txnip), also called Vitamin D3 up-regulated protein 1 (VDUP1) is an endogenous inhibitor of TRX. Redox-dependent regulation of Txnip by mitogenic factors through Reactive oxygen species (ROS) and the specific binding of Txnip to the redox-sensitive cysteine-sulfide center of TRX modulate intracellular levels of ROS and the mitogenic activity of TRX. It has been reported that Txnip plays important roles in diverse cellular processes, including the regulation of cellular redox balance, apoptosis, proliferation, and differentiation.
Related products K0204-3 Anti-Txnip (VDUP1) mAb
M013-3 Anti-Thioredoxin (Trx-tag) mAb
M075-3 Mouse IgG1 (isotype control)
Product category Research area:Cellular stress
Data


Citations
  1. Pang ST et al. Thioredoxin-interacting protein: an oxidative stress-related gene is upregulated by glucose in human prostate carcinoma cells. J Mol Endocrinol. 42, 205-14 (2009)(PMID:19052253)

Western Blotting

  1. Yoshioka J et al. Targeted deletion of thioredoxin-interacting protein regulates cardiac dysfunction in response to pressure overload. Circ Res. 101, 1328-38 (2007)(PMID:17916779)
  2. Chen J et al. Thioredoxin-interacting protein: a critical link between glucose toxicity and beta-cell apoptosis. Diabetes 57, 938-44 (2008)(PMID:18171713)
  3. Chen J et al. Diabetes induces and calcium channel blockers prevent cardiac expression of proapoptotic thioredoxin-interacting protein. Am J Physiol Endocrinol Metab. 296, E1133-9 (2009)(PMID:19258488)
  4. Patwari P et al. Thioredoxin-independent regulation of metabolism by the alpha-arrestin proteins. J Biol Chem. 284, 24996-5003 (2009)(PMID:19605364)
  5. Saxena G et al. Intracellular shuttling and mitochondrial function of thioredoxin-interacting protein. J Biol Chem. 285, 3997-4005 (2010)(PMID:19959470)
  6. Kibbe C et al. FOXO1 competes with carbohydrate response element-binding protein (ChREBP) and inhibits thioredoxin-interacting protein (TXNIP) transcription in pancreatic beta cells. J Biol Chem. 288, 23194-202 (2013)(PMID:23803610)
  7. Jing G et al. Thioredoxin-interacting protein promotes islet amyloid polypeptide expression through miR-124a and FoxA2. J Biol Chem. 289, 11807-15 (2014)(PMID:24627476)
  8. Hong K et al. Cytokines Regulate β-Cell Thioredoxin-interacting Protein (TXNIP) via Distinct Mechanisms and Pathways. J Biol Chem. 291, 8428-39 (2016)(PMID:26858253)
  9. Ahn B et al. MondoA coordinately regulates skeletal myocyte lipid homeostasis and insulin signaling. J Cin Invest. 126, 3567-79 (2016)(PMID:27500491)
  10. Hulmi JJ et al. Effects of muscular dystrophy, exercise and blocking activin receptor IIB ligands on the unfolded protein response and oxidative stress. Free Radic  Biol Med. 99, 308-322 (2016)(PMID:27554968)
  11. Du RH et al. Uncoupling protein 2 modulation of the NLRP3 inflammasome in astrocytes and its implications in depression. Redox Biol. 9, 178-187 (2016)(PMID:27566281)
  12. Coucha M et al. THigh fat diet dysregulates microRNA-17-5p and triggers retinal inflammation: Role of endoplasmic-reticulum-stress. World J Diabetes. 8, 56-65 (2017)(PMID:28265343)
  13. Morita S et al. Targeting ABL-IRE1α Signaling Spares ER-Stressed Pancreatic β Cells to Reverse Autoimmune Diabetes. Cell Metab. 25, 863-897.e8. (2017)(PMID:28380378)
  14. Park S et al. Proteasome inhibitor-induced cleavage of HSP90 is mediated by ROS generation and caspase 10-activation in human leukemic cells. Redox Biol. 13, 470-476 (2017)(PMID:28715732)
  15. Hoshikawa H et al. Combined treatment with D-allose, docetaxel and radiation inhibits the tumor growth in an in vivo model of head and neck cancer. Oncol Lett. 15, 3422-3428 (2018)(PMID:29456721)
  16. Belaya I et al. Long-Term Exercise Protects against Cellular Stresses in Aged Mice. Oxid Med Cell Longev. 2018, 2894247 (2018)(PMID:29765493)
  17. Panse M　et al. Palmitate and insulin counteract glucose-induced thioredoxin interacting protein (TXNIP) expression in insulin secreting cells via distinct mechanisms. PLoS One. 13, e0198016 (2018)(PMID:29813102)
  18. Gunes A et al. Thioredoxin interacting protein promotes invasion in hepatocellular carcinoma. Oncotarget. 9, 36849-36866 (2018) (PMID:30627326)
  19. Danzl K et al. Early inhibition of endothelial retinoid uptake upon myocardial infarction restores cardiac function and prevents cell, tissue, and animal death. J Mol Cell Cardiol. 126 105-117 (2019)(PMID:30472251)
  20. Levring TB et al. Tumor necrosis factor induces rapid down-regulation of TXNIP in human T cells. Sci Rep. 9, 16725 (2019) (PMID:31723203)
  21. Ishibashi T et al. Nicotinic acetylcholine receptor signaling regulates inositol-requiring enzyme 1α activation to protect β-cells against terminal unfolded protein response under irremediable endoplasmic reticulum stress. J Diabetes Investig. 11, 801-813 (2020)(PMID:31925927)

Immunocytochemistry

  1. Jung H et al. Thioredoxin-interacting protein regulates haematopoietic stem cell ageing and rejuvenation by inhibiting p38 kinase activity. Nat Commun. 7, 13674 (2016)(PMID:27929088)

Immunohistochemistry

  1. Saxena G et al. Intracellular shuttling and mitochondrial function of thioredoxin-interacting protein. J Biol Chem. 285, 3997-4005 (2010)(PMID:19959470)
  2. Hand LE et al. Induction of the metabolic regulator Txnip in fasting-induced and natural torpor. Endocrinology 154, 2081-91 (2013)(PMID:23584857)
  3. Morrison JA et al. Thioredoxin interacting protein (TXNIP) is a novel tumor suppressor in thyroid cancer. Mol Cancer. 13, 62 (2014)(PMID:24645981)
  4. Danzl K et al. Early inhibition of endothelial retinoid uptake upon myocardial infarction restores cardiac function and prevents cell, tissue, and animal death. J Mol Cell Cardiol. 126 105-117 (2019) (PMID:30472251 )
  • The availability is based on the information in Japan at 00:05, Apr 20, 2024 in JST.
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  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
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