Antibody detailed product information

Code No.	D365-3
Anti-PIWIL2 (MILI) (Mouse) mAb
Size	100 µL (1 mg/mL)
Availability (in Japan)	10 or more （In Japan at 00:05, Apr 19, 2024 in JST）
Clonality	Monoclonal
Clone	3G5-E6
Isotype (Immunized Animal)	Mouse IgG2aκ
Applications	WB　1 µg/mL IP　2 μg/sample IH　1 µg/mL (Heat treatment is necessary for paraffin embedded sections.)
Reactivity [Gene ID]	Human(-), Mouse[57746], Rat**
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Alternative names	Piwi-like protein 2
Background	PIWIL2 (MILI) is one of three murine PIWI subfamily proteins. They are expressed specifically in gonads, which then form complexes with small RNAs termed piRNAs; and these are responsible for transposon silencing in a mechanism known as “RNA silencing”.
Related products	PM044 Anti-PIWIL2 (MILI) (Mouse) pAb RN003M Anti-EIF2C2 (AGO2) (Human) mAb RN1005 RIP-Assay Kit for microRNA M207-3 Anti-MitoPLD (Pld6) mAb D363-3 Anti-Ly6k (Mouse) mAb D357-3 Anti-Ace (CD143) (Mouse) mAb D356-3 Anti-Jmjd1c (Mouse) mAb
Product category	Research area:Stem cell research RNA-RNP Network Epigenetics Cancer
Data
References	Kuramochi-Miyagawa S et al. Two mouse piwi-related genes: miwi and mili. Mech Dev. 108, 121-33 (2001)（PMID: 11578866） Kuramochi-Miyagawa S et al. Mili, a mammalian member of piwi family gene, is essential for spermatogenesis. Development 131, 839-49 (2004)（PMID: 14736746） Aravin A et al. A novel class of small RNAs bind to MILI protein in mouse testes. Nature 442, 203-7 (2006)（PMID: 16751777） Aravin A et al. Developmentally regulated piRNA clusters implicate MILI in transposon control. Science 316, 744-7 (2007)（PMID: 17446352） Manakov SA et al. MIWI2 and MILI Have Differential Effects on piRNA Biogenesis and DNA Methylation. Cell Rep. 12, 1234-43 (2015)（PMID: 26279574）

The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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