Code No. | D161-3 | |
Anti-MFG-E8 (Mouse) mAb | ||
Size | 100 µL (1 mg/mL) | |
Availability (in Japan) | 10 or more
(In Japan at 17:33, Apr 26, 2024 in JST) |
|
Clonality | Monoclonal | |
Clone | 2422 | |
Isotype (Immunized Animal) |
Hamster IgG | |
Applications | IP 5 µg/250 µL of mouse bone marrow cells culture supernatant FCM 5-10 µg/mL IH* reported. (PMID: 18292292) ELISA* reported. (PMID: 17673941) NT* reported. (PMID: 25006249) |
|
Immunogen (Antigen) |
thioglycollate-elicited mouse peritoneal macrophages | |
Reactivity [Gene ID] | Mouse[17304] |
|
Storage buffer | 1 mg/mL in PBS/50% glycerol, pH 7.2 | |
Storage temp. | -20°C | |
Conjugate | Unlabeled | |
Manufacturer | MBL | |
Alternative names | P47, MP47, Mfgm, SED1, lactadherin | |
Background | Apoptotic cells are rapidly removed by phagocytosis to clear the living cells and tissues of intracellular materials being released from dying cells. Phagocytes recognize apoptotic cells through milk fat globule-EGF-factor 8 (MFG-E8). MFG-E8 is a secreted glycoprotein from thioglycollate-elicited macrophages and bind to phosphatidylserine exposed on the cell surface of apoptotic cells, then bind strongly to cells expressing anb3 integrin via its RGD (arginine-glycine-aspartate) motif. Thus, MFG-E8 is an important protein to mediate the engulfment of apoptotic cells by activated macrophages. | |
Related products | M199-3 Armenian Hamster IgG (isotype control) D199-3 Anti-MFG-E8 (Mouse) mAb SY-001 Anti-Fas (CD95) (Human) mAb |
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Product category | Research area:Apoptosis | |
Data | ||
Citations | Western Blotting
Neutralization
Immunoprecipitation
Flow Cytometry
Immunohistochemistry
ELISA
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