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HOME > Product search results > Code No. RN024P Anti-PCBP1 pAb

Code No. RN024P

Anti-PCBP1 pAb

Availability (in Japan)

0
(Contact us)

(In Japan at 00:05,
Oct 16, 2018 in JST)

Size

200 µg/200 µL

Data
  • Western Blotting

  • Immunoprecipitation

  • RNP Immunoprecipitation

Clonality Polyclonal Clone Polyclonal
Isotype (Immunized Animal) Rabbit Ig (aff.)
Applications
WB
1 µg/mL  
IP
5 µg/500 µL of cell extract from 5 x 106 cells  
IC*
reported.  (PMID: 26990993
RIP
15 µg/500 µL of cell extract from 1.5 x 107 cells  
Other(EMSA)
reported.  (PMID: 27220277
Immunogen (Antigen) KLH-conjugated synthetic peptide, corresponding to internal region of human PCBP1
Reactivity [Gene ID]

Human[5093], Mouse[23983], Rat[500242]

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names poly(rC) binding protein 1, HNRPE1, HNRPX, hnRNP-E1, hnRNP-X
Background Poly(rC)-binding proteins (PCBPs) are cellular RNA-binding proteins; these proteins are defined by their triple KH structure and poly(C)-binding specificity. The 3 KH domains are not only involved in nucleic acid binding but also in mediating protein-protein interactions within the RNP complexes. PCBPs bind specifically to polypyrimidine tracts within the 3′ UTR of target mRNAs through the KH domains. PCBPs are expressed as 4 isoforms in cells. PCBP1 and PCBP2 are widely expressed in both the nucleus and the cytoplasm and can shuttle from the nucleus to the cytoplasm, while PCBP3 and PCBP4 are expressed to a less extent. PCBP1 and PCBP2 have been reported to stabilize the target mRNAs such as those of α-globin, α1(I) collagen, and tyrosine hydroxylase by binding to C-rich 3′ UTR motifs. The 15-lipoxygenase mRNA is translationally silent until reticulocytes in the peripheral blood undergo the final steps of maturation. This translational silencing has been shown to be mediated by the binding of PCBP1 and hnRNPK to the 3′ UTR of 15-lipoxygenase mRNA.
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Citations

Western Blotting

  1. Espinoza-Lewis RA et al. Poly(C)-binding protein 1 (Pcbp1) regulates skeletal muscle differentiation by modulating microRNA processing in myoblasts. J Biol Chem. 292, 9540-9550 (2017)(PMID:28381556)

Immunocytochemistry

  1. Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)

RNP Immunoprecipitation

  1. Yabe-Wada T et al. TLR signals posttranscriptionally regulate the cytokine trafficking mediator sortilin. Sci. Rep. 6, 26566 (2016)(PMID:27220277)

Other(EMSA)

  1. Yabe-Wada T et al. TLR signals posttranscriptionally regulate the cytokine trafficking mediator sortilin. Sci. Rep. 6, 26566 (2016)(PMID:27220277)
Product category
Research area
RNA-RNP Network
Cancer
Brands
RiboCluster Profiler™
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  • The availability is based on the information in Japan at 00:05, Oct 16, 2018 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.