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HOME > Product search results > Code No. RN019M Anti-2,2,7-trimethylguanosine (m3G/TMG) mAb

Code No. RN019M

Anti-2,2,7-trimethylguanosine (m3G/TMG) mAb

Availability (in Japan)

10 or more

(In Japan at 01:30,
Nov 24, 2017 in JST)

Size

200 µg/200 µL

Data
  • Immunoprecipitation

  • Immunocytochemistry

Clonality Monoclonal Clone 235-1
Isotype (Immunized Animal) Mouse IgG2aκ
Applications
IP
10 µg/sample  
IC
0.5-1 µg/mL  
Immunogen (Antigen) Carrier protein-conjugated trimethylguanosine (m3G/TMG)
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names N2,N2,7-Trimethylguanosine, m2,2,7G
Background Small nuclear RNA (snRNA) is known to be involved in pre-mRNA splicing as an RNA component of snRNP. 2,2,7-trimethylated guanosine (m3G/TMG) is a modified nucleoside at the 5’-cap site of U snRNAs except for U6 snRNA. Shortly after transcription, 7-methylguanosine (m7G), called cap structure, is added to 5’-end of newly synthesized U snRNA and m7G-capped U snRNA is exported to the cytoplasm. The m7G-cap is converted to m3G-cap, which is a hypermethylated structure of 5’-end, by cytoplasmic catalytic protein TSG1 and the m3G-capped U snRNA forms snRNP with Sm proteins in the cytoplasm, followed by entry into the nucleus. The m3G-capped snRNP plays a role in regulation of pre-mRNA splicing as a part of spliceosome in nucleus. It is also reported that U3 and U8 snoRNA, localized to Cajal body during RNP biogenesis, possess m3G-cap structure.
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References
  1. Nottrott S et al. Functional interaction of a novel 15.5kD [U4/U6.U5] tri-snRNP protein with the 5' stem-loop of U4 snRNA. EMBO J. 18, 6119-33 (1999)(PMID: 10545122
  2. Nagai K et al. Structure and assembly of the spliceosomal snRNPs. Novartis Medal Lecture. Biochem Soc Trans. 29, 15-26 (2001)(PMID: 11356120
  3. Fox AH et al. Paraspeckles: a novel nuclear domain. Curr Biol. 12, 13-25 (2002)(PMID: 11790299
  4. Yedavalli VS, Jeang KT. Trimethylguanosine capping selectively promotes expression of Rev-dependent HIV-1 RNAs. PNAS 107, 14787-92 (2010)(PMID: 20679221
  5. Tsuiji H et al. Spliceosome integrity is defective in the motor neuron diseases ALS and SMA. EMBO Mol. Med. 5, 221-34 (2013)(PMID: 23255347
Product category
Research area
RNA-RNP Network
Epigenetics
Brands
RiboCluster Profiler™
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  • The availability is based on the information in Japan at 01:30, Nov 24, 2017 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.