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HOME > Product search results > Code No. RN017M Anti-7-methylguanosine (m7G) mAb

Code No. RN017M

Anti-7-methylguanosine (m7G) mAb

Availability (in Japan)

10 or more

(In Japan at 01:30,
Sep 23, 2017 in JST)

Size

200 µg/200 µL

This antibody (clone 4141-13) reacts with both 5′-terminal and internal 7-methylguanosine (m7G) in RNA.
Data
  • Immunoprecipitation

  • Immunoprecipitation

  • Dot Blotting

Clonality Monoclonal Clone 4141-13
Isotype (Immunized Animal) Mouse IgG2aκ
Applications
IP
10 µg/ sample  
IC
Can be used.  
ELISA
Can be used.  
DB
1 µg/mL  
Immunogen (Antigen) Carrier protein-conjugated 7-methylguanosine (m7G)-Cap analogue
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Background The 7-methylguanosine (m7G) is a modified nucleoside commonly found in eukaryotes. The m7G modification present on the 5′-end of mRNA and on some ncRNA synthesized by RNA polymerase II is called m7G cap. It plays a role in transcription, splicing, polyadenylation, nuclear export, stabilization, and translation. In addition, other m7G modifications are also found in the tRNA or rRNA. The m7G46 modification occurs in bacterial and eukaryotic tRNAs. The m7G1575 modification in the 18S rRNA is evolutionarily conserved in eukaryotes.
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References
  1. Maden BE, Salim M. The methylated nucleotide sequences in HELA cell ribosomal RNA and its precursors. J Mol Biol. 88, 133-52 (1974)(PMID: 4374549
  2. Cowling VH. Regulation of mRNA cap methylation. Biochem J. 425, 295-302 (2009)(PMID: 20025612
  3. Cubrilo S et al. The aminoglycoside resistance methyltransferase Sgm impedes RsmF methylation at an adjacent rRNA nucleotide in the ribosomal A site. RNA 15, 1492-7 (2009)(PMID: 19509304
  4. Chen P et al. Transfer RNA modifications and genes for modifying enzymes in Arabidopsis thaliana. BMC Plant Biol. 10, 201 (2010)(PMID: 20836892
  5. Filonava L et al. A novel cause for primordial dwarfism revealed: defective tRNA modification. Genome Biol. 16, 216 (2015)(PMID: 26429597
  6. Zorbas C et al. The human 18S rRNA base methyltransferases DIMT1L and WBSCR22-TRMT112 but not rRNA modification are required for ribosome biogenesis. Mol Biol Cell. 26, 2080-95 (2015)(PMID: 25851604
Product category
Research area
RNA-RNP Network
Epigenetics
Brands
RiboCluster Profiler™
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 01:30, Sep 23, 2017 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.