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HOME > Product search results > Code No. RN014P Anti-TIA1 pAb

Code No. RN014P

Anti-TIA1 pAb

Availability (in Japan)

10 or more

(In Japan at 01:30,
Sep 21, 2017 in JST)

Size

200 µg/200 µL

Data
  • Western Blotting

  • Immunoprecipitation

  • RNP Immunoprecipitation

Clonality Polyclonal Clone Polyclonal
Isotype (Immunized Animal) Rabbit Ig (aff.)
Applications
WB
1:1,000  
IP
5 µg/250 µL of cell extract from 2.5 x 106 cells  
IC
  
RIP
15 µg/500 µL of cell extract from 6 x 106 cells  
Other(eCLIP)
reported.  (PMID: 27018577
Immunogen (Antigen) KLH-conjugated synthetic peptide NGSMLPNQPSGYRVAGYETQ (367-386 aa)
Reactivity [Gene ID]

Human[7072], Mouse[21841], Rat[312510], Hamster

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names TIA1 cytotoxic granule-associated RNA binding protein, WDM,  TIA-1
Background T-cell intracellular antigen-1 (TIA1), an apoptosis promoting factor, is a RNA binding protein which associates with subsets of mRNAs bearing U-rich sequences in their 3’-UTRs and shuttles between nucleus and cytoplasm. TIA1 possesses three RNA recognition motifs (RRMs). RRM2 and the first half of the auxiliary region are necessary for nuclear accumulation whereas RRM3 mediates nuclear export.  TIA1 regulates splicing of alternatively spliced pre-mRNAs including Fas, msl-2, FGFR-2, calsitonin/CGRP in nucleus. In cytoplasm, TIA1 regulates translation of various mRNAs, include TNF-α, Cox-2, HMMP-13 and β2-adrenergic receptor, by binding to AU-rich elements (AREs) located in these mRNA 3’ untranslated regions (3’UTRs). In addition to the translational silencing, TIA1 plays a role in the cellular response to environmental stress by promoting the assembly of a non-canonical 48S preinitiation complex that is the core component of cytoplasmic stress granules (SG). Like other mRNA turnover and translation regulators such as AUF-1, HuR, KSRP and NF90, TIA1 also associates its cognate mRNA and transcripts of other RBPs.
Related products RN1001 RIP-Assay Kit
RN1005 RIP-Assay Kit for microRNA
RN059PW Anti-TIAL1 pAb
RN031PW Anti-ZFP36 (Human) pAb
Citations

Western Blotting

  1. Liu W et al. Hepatitis B virus core protein inhibits Fas-mediated apoptosis of hepatoma cells via regulation of mFas/FasL and sFas expression. FASEB J. 29, 1113-23 (2015)(PMID:25466893)

Other(eCLIP)

  1. Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27018577)
Product category
Research area
RNA-RNP Network
Brands
RiboCluster Profiler™
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 01:30, Sep 21, 2017 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.