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HOME > Product search results > Code No. RN008P Anti-IGF2BP2 (IMP2) pAb

Code No. RN008P

Anti-IGF2BP2 (IMP2) pAb

Availability (in Japan)

10 or more

(In Japan at 01:30,
Nov 23, 2017 in JST)

Size

200 µg/200 µL

Data
  • Western Blotting

  • Immunoprecipitation

  • RNP Immunoprecipitation

Clonality Polyclonal Clone Polyclonal
Isotype (Immunized Animal) Rabbit Ig (aff.)
Applications
WB
1:1,000  
IP
5 µg/250 µL of cell extract from 2.5 x 106 cells  
IC
Can be used.  
RIP
15 µg/500 µL of cell extract from 1.2 x 107 cells  
Other(eCLIP)
reported.  (PMID: 27018577 / 27068461
Other(PAR-CLIP)
reported.  (PMID: 27184842
Immunogen (Antigen) KLH-conjugated synthetic peptide KQQEQKYPQGVASQRSK (583-599 aa)
Reactivity [Gene ID]

Human[10644](22899010), Mouse[319765](22899010), Rat[303824](22513850), Hamster(-)

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names insulin-like growth factor 2 mRNA binding protein 2, p62, IMP-2, VICKZ2/Igf2bp2, Imp2, Neilsen, C330012H03Rik/RGD1305614
Background IGF2BP2 is an RNA binding protein that post-transcriptionally regulates IGF2 during developmental stages. IGF2 mRNA-binding protein family (IGF2BP1, 2 and 3) bind UTR region of IGF2 transcripts and are implicated to in transport of RNA targets to enable protein synthesis at specific locations in the cytoplasm. IGF2BP family members show tissue-selective expression during development. IGF2BP2 is expressed in fetal lung, kidney, thymus, placenta and liver. Genetic valuation in IGF2BP2 was implicated to interact with Fetal Malnutrition on glucose metabolism.
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Citations

Western Blotting

  1. Gong C et al. A long non-coding RNA, LncMyoD, regulates skeletal muscle differentiation by blocking IMP2-mediated mRNA translation. Dev Cell. 34, 181-91 (2015)(PMID:26143994)
  2. Conway AE et al. Enhanced CLIP Uncovers IMP Protein-RNA Targets in Human Pluripotent Stem Cells Important for Cell Adhesion and Survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
  3. Degrauwe N et al. The RNA Binding Protein IMP2 Preserves Glioblastoma Stem Cells by Preventing let-7 Target Gene Silencing. Cell Rep. 15, 1634-47 (2016)(PMID:27184842)

Immunoprecipitation

  1. Janiszewska M et al. Imp2 controls oxidative phosphorylation and is crucial for preserving glioblastoma cancer stem cells. Genes Dev. 26, 1926-44 (2012)(PMID:22899010)
  2. Conway AE et al. Enhanced CLIP Uncovers IMP Protein-RNA Targets in Human Pluripotent Stem Cells Important for Cell Adhesion and Survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
  3. Degrauwe N et al. The RNA Binding Protein IMP2 Preserves Glioblastoma Stem Cells by Preventing let-7 Target Gene Silencing. Cell Rep. 15, 1634-47 (2016)(PMID:27184842)

Immunocytochemistry

  1. Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)
  2. Conway AE et al. Enhanced CLIP Uncovers IMP Protein-RNA Targets in Human Pluripotent Stem Cells Important for Cell Adhesion and Survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)

RNP Immunoprecipitation

  1. Janiszewska M et al. Imp2 controls oxidative phosphorylation and is crucial for preserving glioblastoma cancer stem cells. Genes Dev. 26, 1926-44 (2012)(PMID:22899010)
  2. Schaeffer V et al. RNA-binding protein IGF2BP2/IMP2 is required for laminin-β2 mRNA translation and is modulated by glucose concentration. Am J Physiol Renal Physiol. 303, F75-82 (2012)(PMID:22513850)

Other(eCLIP)

  1. Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27018577)
  2. Conway AE et al. Enhanced CLIP Uncovers IMP Protein-RNA Targets in Human Pluripotent Stem Cells Important for Cell Adhesion and Survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
  3. Van Nostrand EL et al. CRISPR/Cas9-mediated integration enables TAG-eCLIP of endogenously tagged RNA binding proteins. Methods. 118-119, 50-59 (2017)(PMID:28003131)

Other(PAR-CLIP)

  1. Degrauwe N et al. The RNA Binding Protein IMP2 Preserves Glioblastoma Stem Cells by Preventing let-7 Target Gene Silencing. Cell Rep. 15, 1634-47 (2016)(PMID:27184842)
Product category
Research area
RNA-RNP Network
Brands
RiboCluster Profiler™
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 01:30, Nov 23, 2017 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.