Anti-EIF4G2 pAb

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 19, 2024 in JST）

Size

200 µL (1 mg/mL)

Citations

Data Sheet

SDS

Data	Western Blotting Immunoprecipitation RNP Immunoprecipitation
Clonality	Polyclonal	Clone	Polyclonal
Isotype (Immunized Animal)	Rabbit Ig (aff.)
Applications	WB 1:1,000 IP 5 µg/250 µL of cell extract from 2.5 x 10⁶ cells IC* reported. （PMID: 26990993） RIP 15 µg/500 µL of cell extract from 4.5 x 10⁶ cells Other(eCLIP) reported. （PMID: 27018577）
Immunogen (Antigen)	KLH-conjugated synthetic peptide EVNPPSDETDSSSAPSKE (783-800 aa)
Reactivity [Gene ID]	Human[1982], Mouse[13690], Rat[361628], Hamster[100758677]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	eukaryotic translation initiation factor 4 gamma 2, OK/SW-cl.75, AAG1, DAP5, NAT1, P97
Background	The eukaryote polypeptide chain initiation factor eIF4G forms complex with the mRNA cap-binding protein eIF4E and the ATP dependent RNA helicase eIF4A, constituting the initiation factor eIF4F complex. The eIF4G plays an important role in the mechanism of translation by tethering between other components of the ribosomal initiation factors. Two forms of eIF4G have been reported as two different gene products, eIF4G1 and 2. The eIF4G2 was identified as a functional homologue of eIF4G1. The homology of eIF4G2 to eIF4G1 is particularly high in central and carboxyl portions which corresponds to the eIF4A and eIF3 binding region. The proteolysis of both eIF4G1 and eIF4G2 is associated with a decrease in cell viability and the inhibition of protein synthesis.
Related products	RN1001 RIP-Assay Kit RN1005 RIP-Assay Kit for microRNA RN001P Anti-EIF4E pAb RN002P Anti-eIF4G1 (Human) pAb RN004M Anti-Ribosomal P0/P1/P2 mAb RN006M Anti-EIF4E mAb
Citations	Immunocytochemistry Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993) Other(eCLIP) Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27018577)
Product category	Research area RNA-RNP Network Brands RiboCluster Profiler™

The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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