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HOME > Product search results > Code No. RN003M Anti-EIF2C2 (AGO2) (Human) mAb

Code No.　RN003M

Anti-EIF2C2 (AGO2) (Human) mAb

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 20, 2024 in JST）

Size

200 µL (1 mg/mL)

Data

  • Western Blotting

  • Immunoprecipitation

  • RNP Immunoprecipitation
Clonality Monoclonal Clone 1B1-E2H5
Isotype (Immunized Animal) Mouse IgG2aλ
Applications
WB
1-4 µg/mL  
IP
1.5 µg/500 µL of cell extract from 1.5 x 106 cells  
RIP
15 µg/500 µL of cell extract from 1.5 x 107 cells  
Immunogen (Antigen) Recombinant partial EIF2C2 (N-terminal region, approximately 180 a.a.)
Reactivity [Gene ID]

Human[27161], Mouse(-), Rat(-)
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names eukaryotic translation initiation factor 2C2, Q10
Background Eukaryotic translation initiation factor 2C, subunit 2 (EIF2C2; AGO2), is a member of the argonaute protein family. It is a core component of RNA-induced silencing complex (RISC). The EIF2C2 belonging to the human argonaute protein family possesses endonuclease activity and functions as a slicer in gene silencing pathways. It recognizes and cleaves target mRNA by RNA interference. In small interference RNA (siRNA) pathway, EIF2C2 forms a complex with EIF2C1 and siRNA, and in microRNA (miRNA) pathway, it binds EIF2C3 to miRNA. EIF2C2 expression is controlled by epidermal growth factor receptor and mitogen-activated protein kinase signaling in human breast cancer cell line.
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Citations

Western Blotting

  1. Fukao A et al. MicroRNAs Trigger Dissociation of eIF4AI and eIF4AII from Target mRNAs in Humans. Mol Cell 56, 79-89 (2014)(PMID:25280105)
  2. Degrauwe N et al. The RNA Binding Protein IMP2 Preserves Glioblastoma Stem Cells by Preventing let-7 Target Gene Silencing. Cell Rep. 15, 1634-47 (2016)(PMID:27184842)
  3. Yamane D et al. Differential hepatitis C virus RNA target site selection and host factor activities of naturally occurring miR-122 3' variants. Nucleic Acids Res. 45, 4743-4755 (2017)(PMID:28082397)
  4. Cui J, Placzek WJ. PTBP1 enhances miR-101-guided AGO2 targeting to MCL1 and promotes miR-101-induced apoptosis. Cell Death Dis. 9, 552 (2018)(PMID:29748555)

RNP Immunoprecipitation

  1. Zhang LY et al. MicroRNA-144 promotes cell proliferation, migration and invasion in nasopharyngeal carcinoma through repression of PTEN. Carcinogenesis 34, 454-63 (2013)(PMID:23125220)
  2. Comincini S et al. microRNA-17 regulates the expression of ATG7 and modulates the autophagy process, improving the sensitivity to temozolomide and low-dose ionizing radiation treatments in human glioblastoma cells. Cancer Biol Ther. 14, 574-86 (2013)(PMID:23792642)
  3. Marchesi N et al. Autophagy is modulated in human neuroblastoma cells through direct exposition to low frequency electromagnetic fields. J Cell Physiol. 229, 1776-86 (2014)(PMID:24676932)
  4. Selitsky SR et al. Small tRNA-derived RNAs are increased and more abundant than microRNAs in chronic hepatitis B and C. Sci Rep. 5, 7675 (2015)(PMID:25567797)
  5. Lopez P et al. RNA Activation of the Vascular Endothelial Growth Factor Gene (VEGF) Promoter by Double-Stranded RNA and Hypoxia: Role of Noncoding VEGF Promoter Transcripts. Mol Cell Biol. 36, 1480-93 (2016)(PMID:26976645)
  6. Yamane D et al. Differential hepatitis C virus RNA target site selection and host factor activities of naturally occurring miR-122 3' variants. Nucleic Acids Res. 45, 4743-4755 (2017)(PMID:28082397)
  7. Al-Haidari AA et al. MiR-155-5p positively regulates CCL17-induced colon cancer cell migration by targeting RhoA. Oncotarget. 8, 14887-14896 (2017)(PMID:28146427)
  8. Cui J, Placzek WJ. PTBP1 enhances miR-101-guided AGO2 targeting to MCL1 and promotes miR-101-induced apoptosis. Cell Death Dis. 9, 552 (2018)(PMID:29748555)
  9. Bach DH et al. BMP4 Upregulation Is Associated with Acquired Drug Resistance and Fatty Acid Metabolism in EGFR-Mutant Non-Small-Cell Lung Cancer Cells. Mol Ther Nucleic Acids. 12, 817-828 (2018)(PMID:30153566)
  10. Wen J et al. miR-424 coordinates multilayered regulation of cell cycle progression to promote esophageal squamous cell carcinoma cell proliferation. EBioMedicine. 37, 110-124 (2018) (PMID:30361064)
Product category
Research area
RNA-RNP Network
Brands
RiboCluster Profiler™
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 00:05, Apr 20, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.