Anti-EEA1 pAb

Availability (in Japan)

1-9

（In Japan at 00:05,
Apr 19, 2024 in JST）

Size

100 µL

Citations

Data Sheet

SDS

Data	Western Blotting Immunoprecipitation Immunocytochemistry
Clonality	Polyclonal	Clone	Polyclonal
Isotype (Immunized Animal)	Rabbit Ig (aff.)
Applications	WB 1:1,000 IP 2 µL/300 µL of cell extract from 1 x 10⁷ cells IC 1:200
Immunogen (Antigen)	N-terminal EEA1 synthetic peptide
Reactivity [Gene ID]	Human[8411], Mouse[216238], Rat[314764]
Storage buffer	PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Background	The early endosome is a cellular compartment inside eukaryotic cells. It is receiving endocytosed material and sorting them for vesicular transport to late endosomes and lysosomes or for recycling to the plasma membrane. EEA1 (Early Endosome Antigen1) is a 170 kDa coiled-coil protein, which is required for vesicular transport of proteins through early endosomes. It binds membrane lipids through its FYVE domain.
Related products	M179-3 Anti-GM130 mAb M176-3 Anti-EEA1 mAb M178-3 Anti-Calnexin mAb PM064 Anti-Lamin B1 pAb M181-3 Anti-KDEL mAb PM059 Anti-KDEL pAb PM061 Anti-GM130 pAb PM063 Anti-COX4 pAb M176-A48 Anti-EEA1 mAb-Alexa Fluor™ 488
Citations	Immunocytochemistry Abe S et al. Localization of Protein Kinase NDR2 to Peroxisomes and Its Role in Ciliogenesis. J Biol Chem. 292, 4089-4098 (2017)(PMID:28122914) Ishii S et al. Identification of a factor controlling lysosomal homeostasis using a novel lysosomal trafficking probe. Sci Rep. 9, 11635 (2019)(PMID:31406169) Immunofluorescence Watanabe S et al. Zinc regulates ERp44-dependent protein quality control in the early secretory pathway. Nat Commun. 10, 603 (2019)(PMID:30723194) Naoto Matsumoto N et al. PP1C and PP2A are p70S6K Phosphatases Whose Inhibition Ameliorates HLD12-Associated Inhibition of Oligodendroglial Cell Morphological Differentiation. Biomedicines. 8, 89 (2020)(PMID:32316234)
Product category	Tools Others

The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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