Anti-Oct3/4 pAb

Availability (in Japan)

1-9

（In Japan at 17:00,
Apr 16, 2024 in JST）

Size

100 µL

Citations

Data Sheet

SDS

Data	Western Blotting Immunoprecipitation Immunocytochemistry Immunohistochemistry
Clonality	Polyclonal	Clone	polyclonal
Isotype (Immunized Animal)	Rabbit Ig (aff.)
Applications	WB 1:1,000 IP 2 µL/300 µL of cell extract from 3 x 10⁶ cells IC IH 1:500
Immunogen (Antigen)	Recombinant Mouse Oct3/4 N-terminal （1-134 aa)
Reactivity [Gene ID]	Human[5460], Mouse[18999]
Storage buffer	PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	POU5F1, OCT3, OCT4, OTF3, OTF4, OTF-3
Background	The POU family transcription factor Oct-3/4, termed as Oct-3 or Oct-4, encoded by Pou5f1, is expressed in totipotent/pluripotent early embryonic cells. It is also expressed in embryonic stem (ES) cells and embryonal carcinoma (EC) cells, but its expression diminishes when these cells differentiate and lose pluripotency. Oct3/4 contains three functionally characterized domains, the transcriptional activation domain of N- and C-terminal region and the POU DNA-binding domain. The POU domain binds to an octamer sequence, ATTTGCAT. Several target genes of Oct3/4, such as Sox2, contains an octamer element capable of binding Oct3/4. These sites are important for transcriptional activity. Induced pluripotent stem (iPS) cells can be generated from mouse embryonic or adult fibroblasts by induction of four factors, Oct3/4, Sox2, c-Myc, and Klf4. Oct3/4 regulates a expression of Tcl1 (T cell lymphoma break point) and Nanog and contributes to cell proliferation and stabilization of cell pluripotency. Two transcription factors Oct3/4 and Sox2 works together to control a transcriptional regulatory network that regulates the expression of other essential genes.
Related products	D356-3 Anti-Jmjd1c (Mouse) mAb PM056 Anti-Sox2 pAb PM057 Anti-KLF4 pAb PM058 Anti-Nanog (Mouse) pAb
Citations	Immunocytochemistry Tsukiyama T, Ohinata Y. A modified EpiSC culture condition containing a GSK3 inhibitor can support germline-competent pluripotency in mice. PLoS One 9, e95329 (2014)(PMID:24736627) Oura S et al. Chimeric analysis with newly established EGFP/DsRed2-tagged ES cells identify HYDIN as essential for spermiogenesis in mice. Exp Anim. (2018) In press.(PMID:30089752) Immunohistochemistry Watanabe Y et al. Notch and Hippo signaling converge on Strawberry Notch 1 (Sbno1) to synergistically activate Cdx2 during specification of the trophectoderm. Sci Rep. 7, 46135 (2017)(PMID:28401892)
Product category	Research area Stem cell research

The availability is based on the information in Japan at 17:00, Apr 16, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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