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HOME > Product search results > Code No. MI-11-3 Anti-Bromodeoxyuridine mAb

Code No. MI-11-3

Anti-Bromodeoxyuridine mAb

Availability (in Japan)

10 or more

(In Japan at 01:30,
Nov 23, 2017 in JST)

Size

100 µg/100 µL

Data
  • Immunohistochemistry

Clonality Monoclonal Clone 2B1
Isotype (Immunized Animal) Mouse IgG1
Applications
IP*
DNA-IP (PMID: 22850674/18708580/12944972/17671091)  RNA-IP (PMID: 22369889/23064114/23383264)  
FCM
10 µg/mL (final concentration)  
IC*
reported.  (PMID: 19103807
IH
10 µg/mL  
Immunogen (Antigen) 5-Iodouridine-Ovalbumin
Reactivity [Gene ID]

Human

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names BrdU, 5-Bromo-2-Deoxyuridine
Background BrdU (5-Bromo-2-Deoxyuridine) is a derivative of uridine that can substitute for thymidine during DNA synthesis. The detection of BrdU incorporation into DNA is a common method to quantify newly synthesized DNA and to identify cells in the S-phase of the cell cycle. BrdU incorporation is frequently used in proliferation assays to study DNA repair, sister chromatid exchange, and the cytokinetics of normal and neoplastic cells.
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D345-3 Anti-1-methyladenosine (m1A) mAb
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RN131P Anti-N6-methyladenosine (m6A) pAb
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RN016M Anti-7-methylguanosine (m7G)-Cap mAb
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Citations

Immunoprecipitation

  1. Katou Y et al. S-phase checkpoint proteins Tof1 and Mrc1 form a stable replication-pausing complex. Nature 424,1078-83 (2003)(PMID:12944972)
  2. Bermejo R et al. Top1- and Top2-mediated topological transitions at replication forks ensure fork progression and stability and prevent DNA damage checkpoint activation. Genes Dev. 21, 1921-36 (2007)(PMID:17671091)
  3. D'Ambrosio C et al. Identification of cis-acting sites for condensin loading onto budding yeast chromosomes. Genes Dev. 22, 2215-27 (2008)(PMID:18708580)
  4. Tani H et al. Genome-wide determination of RNA stability reveals hundreds of short-lived noncoding transcripts in mammals. Genome Res. 22, 947-56 (2012)(PMID:22369889)
  5. Yamazaki S et al. Rif1 regulates the replication timing domains on the human genome. EMBO J. 31, 3667-77 (2012)(PMID:22850674)
  6. Tani H et al. Identification of hundreds of novel UPF1 target transcripts by direct determination of whole transcriptome stability. RNA Biol. 9, 1370-9 (2012)(PMID:23064114)
  7. Alzu A et al. Senataxin associates with replication forks to protect fork integrity across RNA-polymerase-II-transcribed genes. Cell 151, 835-46 (2012)(PMID:23141540)
  8. Tani H et al. The RNA degradation pathway regulates the function of GAS5 a non-coding RNA in mammalian cells. PLoS One 8, e55684 (2013)(PMID:23383264)
  9. Gonzalez-Huici V et al. DNA bending facilitates the error-free DNA damage tolerance pathway and upholds genome integrity. EMBO J. 33, 3667-77 (2014)(PMID:24473148)
  10. Toteva T et al. Establishment of expression-state boundaries by Rif1 and Taz1 in fission yeast. PNAS 114, 1093-1098 (2017)(PMID:28096402)

Flow Cytometry

  1. Nakajima T et al. Suppression of adenovirus E1A-induced apoptosis by mutated p53 is overcome by coexpression with Id proteins. PNAS. 95,10590-5 (1998)(PMID:9724748)
  2. Liu Q et al. Elevated cyclin E levels, inactive retinoblastoma protein, and suppression of the p27(KIP1) inhibitor characterize early development of promyeloid cells into macrophages. Mol Cell Biol. 19, 6229-39 (1999)(PMID:10454569)

Immunocytochemistry

  1. Ito S et al. Epstein-Barr virus nuclear antigen-1 is highly colocalized with interphase chromatin and its newly replicated regions in particular.J Gen Virol. 83, 2377-83 (2002)(PMID:12237418)
  2. Itakura E et al. Dimerization of the ATRIP protein through the coiled-coil motif and its implication to the maintenance of stalled replication forks. Mol Biol Cell 16, 5551-62 (2005)(PMID:16176973)
  3. Sugimura K et al. PARP-1 ensures regulation of replication fork progression by homologous recombination on damaged DNA. J Cell Biol. 183, 1203-12 (2008)(PMID:19103807)
  4. Yamazaki S et al. Rif1 regulates the replication timing domains on the human genome. EMBO J. 31, 3667-77 (2012)(PMID:22850674)

Immunohistochemistry

  1. Imai F et al. Inactivation of aPKClambda results in the loss of adherens junctions in neuroepithelial cells without affecting neurogenesis in mouse neocortex. Development. 133, 1735-44 (2006)(PMID:16571631)
  2. Yeom SY et al. Regulation of insulin secretion and beta-cell mass by activating signal cointegrator 2. Mol Cell Biol. 26, 4553-63 (2006)(PMID:16738321)
  3. Hirai S et al. The c-Jun N-terminal kinase activator dual leucine zipper kinase regulates axon growth and neuronal migration in the developing cerebral cortex. J Neurosci. 26, 11992-2002 (2006)(PMID:17108173)
  4. Yamashita N et al. Collapsin response mediator protein 1 mediates reelin signaling in cortical neuronal migration. J Neurosci. 26, 13357-62 (2006)(PMID:17182786)
  5. Chen Q et al. EphA4 Regulates the Balance between Self-Renewal and Differentiation of Radial Glial Cells and Intermediate Neuronal Precursors in Cooperation with FGF Signaling. PLoS One 10, e0126942 (2015)(PMID:25978062)
  6. Wang D et al. spalt is functionally conserved in Locusta and Drosophila to promote wing growth. Sci Rep. 7, 44393 (2017)(PMID:28300136)

Other

  1. D'Ambrosio C et al. Identification of cis-acting sites for condensin loading onto budding yeast chromosomes. Genes Dev. 22, 2215-27 (2008)(PMID:18708580)
Product category
Research area
RNA-RNP Network
Epigenetics
Cancer
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 01:30, Nov 23, 2017 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.