Anti-NFIL3 (E4BP4) chimeric mAb

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 18, 2024 in JST）

Size

100 µL (1 mg/mL)

Citations

Data Sheet

SDS

This antibody is a chimeric antibody composed of guinea pig Ig κ and rabbit Fc. Please use anti-rabbit IgG antibody as a secondary antibody.

Data	Western Blotting Western Blotting Immunoprecipitation Immunoprecipitation Flow Cytometry Immunohistochemistry
Clonality	Monoclonal	Clone	42
Isotype (Immunized Animal)	Guinea pig Ig κ (chimeric)
Applications	WB 1 -2 µg/mL IP 1 -2 µg/sample FCM 1 µg/mL IH Can be used.
Immunogen (Antigen)	Recombinant protein, corresponding to internal region of mouse Nfil3 (E4bp4)
Reactivity [Gene ID]	Human[4783], Mouse[18030], Rat(-)
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	IL3BP1, NFIL3A, NF-IL3A
Background	NFIL3, or E4BP4, is a transcription factor that controls sleep and circadian rhythms throughout the cranial nerve, endocrine, and metabolic systems.The circadian clock is a 24-hour endogenous oscillator, which cycles via transcriptional/translational feedback loops of clock genes and their products in individual cells. CLOCK and BMAL1 are positive factors in the loop, and their complex binds to E-box elements of PERs and CRYs. PER and CRY bind the CLOCK-BMAL1 complex, suppressing E-box-dependent transactivation and forming a molecular clock via this negative feedback mechanism.D-box and REV-ERB/ROR-binding elements also form a regulatory network of clock-controlled genes (CCGs). D-box-dependent transactivation is activated by DBP and repressed by NFIL3/E4BP4.NFIL3/E4BP4 expression also plays an important role in the immune system. NFIL3 polymorphisms are associated with inflammatory bowel disease (IBD).
Related products	D335-3 Anti-BMAL1 mAb D334-3 Anti-CLOCK (Mouse) mAb D333-3 Anti-CLOCK (Mouse) mAb PM079 Anti-DBP (Mouse) pAb PM075 Anti-GNAT2 (Zebrafish) pAb PM081 Anti-Cry1 (Mouse) pAb PM082 Anti-Cry2 (Mouse) pAb PM083 Anti-Per2 (Mouse) pAb PM087 Anti-Chrono (Mouse) pAb PM091 Anti-Per1 (Mouse) pAb PM092 Anti-NR1D1 (Rev-erbα) pAb PM093 Anti-NR1D2 (Rev-erbβ) pAb D349-3 Anti-CLOCK mAb M219-3 Anti-RORγt mAb PM096 Anti-PER2 (Human) pAb PM097 Anti-NFIL3 (E4BP4) pAb
Notes	*Zeitgeber time: Mice were maintained in a light-dark (LD) cycle (12 hr light, 12 hr dark with lights on time was designatedas Zeitgeber time (ZT) 0 and light off time was designated as ZT12).
Citations	Western Blotting Yu F et al. The Circadian Clock Gene Bmal1 Controls Intestinal Exporter MRP2 and Drug Disposition. Theranostics. 9, 2754-2767 (2019)(PMID:31244920)
References	Yu X et al. Th17 cell differentiation is regulated by the circadian clock. Science. 342, 727-30 (2013)（PMID: 24202171） Firth MA et al. Nfil3-independent lineage maintenance and antiviral response of natural killer cells. J Exp Med. 210, 2981-90 (2013) （PMID: 24277151） Kashiwada M et al. IL-4-induced transcription factor NFIL3/E4BP4 controls IgE class switching. PNAS 107, 821-6 (2010)（PMID: 20080759） Mitsui S et al. Antagonistic role of E4BP4 and PAR proteins in the circadian oscillatory mechanism. Genes Dev. 15, 995-1006 (2001)（PMID: 11316793） Ikushima S et al. Pivotal role for the NFIL3/E4BP4 transcription factor in interleukin 3-mediated survival of pro-B lymphocytes. PNAS 94, 2609-14 (1997)（PMID: 9122243） Zhang W et al. Molecular cloning and characterization of NF-IL3A, a transcriptional activator of the human interleukin-3 promoter. Mol Cell Biol. 15, 6055–63 (1995)（PMID: 7565758） Cowell IG et al. Transcriptional repression by a novel member of the bZIP family of transcription factors. Mol Cell Biol. 12, 3070-7 (1992)（PMID: 1620116）
Product category	Research area Immunology Circadian rhythm

The availability is based on the information in Japan at 00:05, Apr 18, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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