Anti-LC3 mAb for My select sampler set

Availability (in Japan)

1-9

（In Japan at 00:05,
Apr 24, 2024 in JST）

Size

20 µL (2 mg/mL)

Citations

Data Sheet

SDS

Data	Western Blotting Immunoprecipitation
Clonality	Monoclonal	Clone	4E12
Isotype (Immunized Animal)	Mouse IgG1 κ
Applications	WB 5 µg/mL IP 5 µg/300 µL of cell extract from 1x10⁷ cells FCM 40 µg/mL (final concentration) IC 40 µg/mL IH* reported. （PMID: 21957238 / 19955365 / 19915056） Other(Immuno-EM) 20 µg/mL
Immunogen (Antigen)	Recombinant Human LC3 (MAP1LC3B: 1-120 a.a.)
Reactivity [Gene ID]	Human[81631/84557], Mouse[67443/66734], Rat[64862/362245], Hamster[100760576/100769810]
Storage buffer	2 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	microtubule-associated protein 1 light chain 3 beta, microtubule-associated protein 1 light chain 3 alpha, LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1LC3B, LC3A, ATG8E, MAP1ALC3, MAP1BLC3, MAP1LC3A
Background	Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The rat microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I. A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II. This antibody can detect both forms of LC3.
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Citations	Western Blotting Ho H et al. WIPI1 coordinates melanogenic gene transcription and melanosome formation via TORC1 inhibition. J Biol Chem. 286, 12509-23 (2011)(PMID:21317285) Saiki S et al. Caffeine induces apoptosis by enhancement of autophagy via PI3K/Akt/mTOR/p70S6K inhibition. Autophagy 7, 176-187 (2011)(PMID:21081844) Kasai M et al. Autophagic compartments gain access to the MHC class II compartments in thymic epithelium. J Immunol. 183, 7278-85 (2009)(PMID:19915056) Wang Y et al. Loss of macroautophagy promotes or prevents fibroblast apoptosis depending on the death stimulus. J Biol Chem. 283,　4766-77 (2008)(PMID:18073215) Murthy A et al. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature 506, 456-62 (2014)(PMID:24553140) Immunoprecipitation Mookerjee S et al. Posttranslational modification of ataxin-7 at lysine 257 prevents autophagy-mediated turnover of an N-terminal caspase-7 cleavage fragment. J Neurosci. 29, 15134-44 (2009)(PMID:19955365) Immunocytochemistry Kasai M et al. Autophagic compartments gain access to the MHC class II compartments in thymic epithelium. J Immunol. 183, 7278-85 (2009)(PMID:19915056) Nishitsuji K et al. The E693Delta mutation in amyloid precursor protein increases intracellular accumulation of amyloid beta oligomers and causes endoplasmic reticulum stress-induced apoptosis in cultured cells. Am J Pathol. 174, 957-69 (2009)(PMID:19164507) Wang Y et al. Loss of macroautophagy promotes or prevents fibroblast apoptosis depending on the death stimulus. J Biol Chem. 283,　4766-77 (2008)(PMID:18073215) Chalazonitis A et al. Homeodomain interacting protein kinase 2 regulates postnatal development of enteric dopaminergic neurons and glia via BMP signaling. J Neurosci. 31, 13746-57 (2011)(PMID:21957238) Mori H et al. Induction of selective autophagy in cells replicating hepatitis C virus genome. J Gen Virol. 99, 1643-1657 (2018)(PMID:30311874) Immunohistochemistry Kasai M et al. Autophagic compartments gain access to the MHC class II compartments in thymic epithelium. J Immunol. 183, 7278-85 (2009)(PMID:19915056) Mookerjee S et al. Posttranslational modification of ataxin-7 at lysine 257 prevents autophagy-mediated turnover of an N-terminal caspase-7 cleavage fragment. J Neurosci. 29, 15134-44 (2009)(PMID:19955365) Other(Immuno-EM) Murthy A et al. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature 506, 456-62 (2014)(PMID:24553140)
Product category	Research area Autophagy

The availability is based on the information in Japan at 00:05, Apr 24, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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