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HOME > Product search results > Code No. D347-3 Anti-Pseudouridine mAb

Code No. D347-3

Anti-Pseudouridine mAb

Availability (in Japan)

10 or more

(In Japan at 01:30,
Sep 21, 2017 in JST)

Size

100 µg/100 µL

Data
  • Immunocytochemistry

  • Immunohistochemistry

Clonality Monoclonal Clone APU-6
Isotype (Immunized Animal) Mouse IgG1 κ
Applications
IC
1 µg/mL  
IH
1 µg/mL  
ELISA*
reported.  (PMID: 2758683 / 8252470
Other(Immuno-Nothern blotting)
Can be used.  (PMID: 26606401
Immunogen (Antigen) KLH-conjugated pseudouridine
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names 5-β-D-Ribofuranosyluracil, beta-pseudouridine, Ψ
Background Pseudouridine (ψ), the first modified RNA base to be identified, presents mostly in non-coding RNAs such as tRNA, rRNA, snRNA and snoRNA. It is also reported that pseudouridine has been found in mRNA in both yeast and human by sequencing analysis. Replacing uridine to pseudouridine enhances structural stability of RNA, and is expected to affect rRNA processing, translation and pre-mRNA splicing.
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Citations

Other(Immuno-Nothern blotting)

  1. Mishima E et al. Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides. PLoS One 10, e0143756 (2015)(PMID:26606401)
References
  1. Masuda M et al. An immunohistochemical analysis for cancer of the esophagus using monoclonal antibodies specific for modified nucleosides. Cancer 72, 3571-8 (1993)  [IHC-P, ELISA](PMID: 8252470
  2. Itoh K et al. Detection of elevated amounts of urinary pseudouridine in cancer patients by use of a monoclonal antibody. Clin Chim Acta 181, 305-15 (1989)  [ELISA](PMID: 2758683
  3. Itoh K et al. Diagnostic use of anti-modified nucleoside monoclonal antibody. Tohoku J Exp Med. 168, 329-31 (1992)(PMID: 1306318
Product category
Research area
RNA-RNP Network
Epigenetics
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 01:30, Sep 21, 2017 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.