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HOME > Product search results > Code No. D345-3 Anti-1-methyladenosine (m1A) mAb

Code No. D345-3

Anti-1-methyladenosine (m1A) mAb

Availability (in Japan)

10 or more

(In Japan at 01:30,
Nov 23, 2017 in JST)

Size

100 µg/100 µL

Data
  • Immunoprecipitation

  • Immunocytochemistry

  • Immunohistochemistry

Clonality Monoclonal Clone AMA-2
Isotype (Immunized Animal) Mouse IgG2bκ
Applications
IP
15 µg/sample  
IC
1 µg/mL  
IH
0.1 µg/mL  
ELISA**
Inhibition ELISA (Info. from the licenser. Not tested by MBL.)  
DB*
reported.  (PMID: 26863196 / 26863410
Other(Immuno-Nothern blotting)
  (PMID: 26606401
Immunogen (Antigen) KLH-conjugated 1-methyladenosine (KLH-m1A)
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names m1Ado
Background In eukaryote, newly synthesized RNA is chemically modified at specific nucleotide position after transcription. N1-methyladenosine (m1A) is one of major modified nucleosides present in most eukaryotic tRNAs and also found in yeast rRNAs. m1A affects structural stability of tRNA and its binding to polysome in translation. Urinary excretion of modified nucleosides including m1A is known to be increased in the patients with various cancers and AIDS. It is reported that the amount of urinary m1A correlates with m1A level in tissue from same patients with esophageal carcinoma. Moreover, elevated serum levels of m1A are also detected under stress conditions.
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Citations

Dot Blotting

  1. Dominissini D et al. The dynamic N(1)-methyladenosine methylome in eukaryotic messenger RNA. Nature 530, 441-6 (2016)(PMID:26863196)
  2. Li X et al. Transcriptome-wide mapping reveals reversible and dynamic N(1)-methyladenosine methylome. Nat Chem Biol. 12, 311-6 (2016)(PMID:26863410)
  3. Liu F et al. ALKBH1-Mediated tRNA Demethylation Regulates Translation. Cell 167, 816-828.e16. (2016)(PMID:27745969)
  4. Ueda Y et al. AlkB homolog 3-mediated tRNA demethylation promotes protein synthesis in cancer cells. Sci Rep. 7, 42271 (2017)(PMID:28205560)

Immunoprecipitation

  1. Dominissini D et al. The dynamic N(1)-methyladenosine methylome in eukaryotic messenger RNA. Nature 530, 441-6 (2016)(PMID:26863196)
  2. Li X et al. Transcriptome-wide mapping reveals reversible and dynamic N(1)-methyladenosine methylome. Nat Chem Biol. 12, 311-6 (2016)(PMID:26863410)
  3. Waku et al. NML-mediated rRNA base methylation links ribosomal subunit formation to cell proliferation in a p53-dependent manner. J. Cell Sci. 129, 2382-93 (2016)(PMID:27149924)

Immunocytochemistry

  1. Ueda Y et al. AlkB homolog 3-mediated tRNA demethylation promotes protein synthesis in cancer cells. Sci Rep. 7, 42271 (2017)(PMID:28205560)

Other(Immuno-Nothern blotting)

  1. Mishima E et al. Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides. PLoS One 10, e0143756 (2015)(PMID:26606401)
References
  1. Itoh K et al. Preparation of a monoclonal antibody specific for 1-methyladenosine and its application for the detection of elevated levels of 1-methyladenosine in urines from cancer patients. Jpn J Cancer Res. 79, 1130-8 (1988)(PMID: 3143701
  2. Itoh K et al. Diagnostic use of anti-modified nucleoside monoclonal antibody. Tohoku J Exp Med. 168, 329-31 (1992)1306318(PMID: 1306318
  3. Masuda M et al. An immunohistochemical analysis for cancer of the esophagus using monoclonal antibodies specific for modified nucleosides.Cancer 72, 3571-8 (1993)(PMID: 8252470
  4. Mishima E et al. Conformational Change in Transfer RNA Is an Early Indicator of Acute Cellular Damage. J Am Soc Nephrol. 25, 2316-26 (2014)(PMID: 24833129
  5. Chi KR. The RNA code comes into focus. Nature 542, 503-506 (2017)(PMID: 28230128
Product category
Research area
RNA-RNP Network
Epigenetics
Cancer
Cellular stress
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 01:30, Nov 23, 2017 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.