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HOME > Product search results > Code No. RN019P Anti-HNRNPK pAb

Code No.　RN019P

Anti-HNRNPK pAb

Availability (in Japan)

1-9

（In Japan at 17:00,
Apr 19, 2024 in JST）

Size

200 µL (1 mg/mL)

Data

  • Western Blotting

  • Immunocytochemistry

  • RNP Immunoprecipitation
Clonality Polyclonal Clone Polyclonal
Isotype (Immunized Animal) Rabbit Ig (aff.)
Applications
WB
1 µg/mL  
IP
5 µg/500 µL of cell extract from 5 x 106 cells  
IC*
reported.  （PMID: 24990929 / 26990993）
RIP
15 µg/500 µL of cell extract from 1.5 x 107 cells  
Other(eCLIP)
reported.  （PMID: 27018577）
Immunogen (Antigen) KLH conjugated synthetic peptide, corresponding to C-terminus of human HNRNPK
Reactivity [Gene ID]

Human[3190], Mouse[15387], Rat[117282]
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names heterogeneous nuclear ribonucleoprotein K, RP11-575L7.1, CSBP, HNRPK, TUNP
Background The heterogeneous nuclear ribonucleoprotein K (hnRNP K), is an RNA-binding protein containing 3 KH RNA-binding domains. It binds to both RNA and DNA and is a component of the hnRNP complex. hnRNP K is associated with pre-mRNA and appears to influence the processing of pre-mRNAs and metabolism of mRNA. hnRNP K is predominantly localized at the nucleus and shuttles between the nucleus and the cytoplasm. Therefore, hnRNP K is thought to be involved in the transport of mRNA. Phosphorylation of hnRNP K leads to its cytoplasmic accumulation and is important for cell migration and metastasis. In the cytoplasm, hnRNP K functions as a translational regulator of specific mRNAs such as c-myc mRNA, 16L2 mRNA, and r15-LOX mRNA. Further, it specifically associates with c-src kinase, leading to c-src activation and hnRNP K phosphorylation, which affect the binding of hnRNP K to RNA.
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Citations

Western Blotting

  1. Folci A et al. Loss of hnRNP K impairs synaptic plasticity in hippocampal neurons. J Neurosci. 34, 9088-95 (2014)(PMID:24990929)
  2. Chung BM et al. Regulation of C-X-C chemokine gene expression by keratin 17 and hnRNP K in skin tumor keratinocytes.　J Cell Biol. 208, 613-27 (2015)(PMID:25713416)

Immunoprecipitation

  1. Chung BM et al. Regulation of C-X-C chemokine gene expression by keratin 17 and hnRNP K in skin tumor keratinocytes.　J Cell Biol. 208, 613-27 (2015)(PMID:25713416)

Immunocytochemistry

  1. Folci A et al. Loss of hnRNP K impairs synaptic plasticity in hippocampal neurons. J Neurosci. 34, 9088-95 (2014)(PMID:24990929)
  2. Chung BM et al. Regulation of C-X-C chemokine gene expression by keratin 17 and hnRNP K in skin tumor keratinocytes.　J Cell Biol. 208, 613-27 (2015)(PMID:25713416)
  3. Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)

RNP Immunoprecipitation

  1. Chung BM et al. Regulation of C-X-C chemokine gene expression by keratin 17 and hnRNP K in skin tumor keratinocytes.　J Cell Biol. 208, 613-27 (2015)(PMID:25713416)
  2. Lubelsky Y, Ulitsky I. Sequences enriched in Alu repeats drive nuclear localization of long RNAs in human cells. Nature 555, 107-111 (2018)(PMID:29466324)

Other(eCLIP)

  1. Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27018577)
Product category
Research area
RNA-RNP Network
Brands
RiboCluster Profiler™
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  • The availability is based on the information in Japan at 17:00, Apr 19, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.