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HOME > Product search results > Code No. PM054 Anti-α-Tubulin pAb

Code No. PM054

Anti-α-Tubulin pAb

Availability (in Japan)

10 or more

(In Japan at 23:00,
Apr 23, 2018 in JST)

Size

100 µL

Data
  • Western Blotting

  • Immunoprecipitation

  • Immunocytochemistry

Clonality Polyclonal Clone Polyclonal
Isotype (Immunized Animal) Rabbit Ig (aff.)
Applications
WB
1:1,000  
IP
2 µL/200 µL of cell extract from 2 x 106 cells  
IC
1:200  
Immunogen (Antigen) N-terminal α-Tubulin synthetic peptide
Reactivity [Gene ID]

Human[7846], Mouse[22142], Rat[64158], Hamster[100682527], Chicken[416694], Fruit fly*(26718004/27559081/28972163)

Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Background Microtubules are one of the components of the cytoskeleton, which performs essential and diverse functions within eukaryotic cells. Microtubules are composed of a heterodimer of alpha and beta tubulins. Tubulin is a GTP-binding protein, and extension and shortening of the microtubules are regulated by binding / hydrolysis of GTP.
Related products PM054-7 Anti-α-Tubulin pAb-HRP-DirecT
PM088 Anti-Vinculin pAb
M175-3 Anti-α-Tubulin mAb
M177-3 Anti-β-Actin mAb
Citations

Western Blotting

  1. Geng Y et al. Contribution of the C-terminal Regions of Promyelocytic Leukemia Protein (PML) Isoforms II and V to PML Nuclear Body Formation. J Biol Chem. 287, 30729-42 (2012)(PMID:22773875)
  2. Cao JX et al. MiR-630 inhibits proliferation by targeting CDC7 kinase, but maintains the apoptotic balance by targeting multiple modulators in human lung cancer A549 cells. Cell Death Dis. 5,e1426  (2014)(PMID:25255219)
  3. Horii T et al. p53 suppresses tetraploid development in mice. Sci Rep. 5, 8907 (2015)(PMID:25752699)
  4. Xu D et al. Modification of BECN1 by ISG15 plays a crucial role in autophagy regulation by type I IFN/interferon. Autophagy 11, 617-28 (2015)(PMID:25906440)
  5. Xu D et al. Phosphorylation and activation of ubiquitin-specific protease-14 by Akt regulates the ubiquitin-proteasome system. Elife 4, e10510 (2015)(PMID:26523394)
  6. Wang H et al. Antagonistic roles of Nibbler and Hen1 in modulating piRNA 3' ends in Drosophila. Development 143, 530-9 (2016)(PMID:26718004)
  7. Li H et al. Ultra-deep sequencing of ribosome-associated poly-adenylated RNA in early Drosophila embryos reveals hundreds of conserved translated sORFs. DNA Res. 23, 571-580 (2016)(PMID:27559081)
  8. Xu D et al. USP25 regulates Wnt signaling by controlling the stability of tankyrases. Genes Dev. 31, 1024-1035 (2017)(PMID:28619731)
  9. Shibutani M et al. Arid1b Haploinsufficiency Causes Abnormal Brain Gene Expression and Autism-Related Behaviors in Mice. Int J Mol Sci. 18, E1872 (2017)(PMID:28867767)
  10. Murakami A et al. An N-terminal di-proline motif is essential for fatty acid-dependent degradation of Δ9-desaturase in Drosophila. J Biol Chem. 292, 19976-19986 (2017)(PMID:28972163)
  11. Fu W et al. A novel acridine derivative, LS-1-10 inhibits autophagic degradation and triggers apoptosis in colon cancer cells. Cell Death Dis. 8, e3086 (2017)(PMID:28981103)

Immunocytochemistry

  1. Taniuchi K et al. KIF20A-mediated RNA granule transport system promotes the invasiveness of pancreatic cancer cells. Neoplasia 16, 1082-93 (2014)(PMID:25499221)
  2. Chinen T et al. The γ-tubulin-specific inhibitor gatastatin reveals temporal requirements of microtubule nucleation during the cell cycle. Nat Commun. 6, 8722 (2015)(PMID:26503935)
  3. Ma K et al. PTK2-mediated degradation of ATG3 impedes cancer cells susceptible to DNA damage treatment. Autophagy 13, 579-591 (2017)(PMID:28103122)
Product category
Tools
Control antibodies
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  • The availability is based on the information in Japan at 23:00, Apr 23, 2018 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.