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HOME > Product search results > Code No. PM020 Anti-DDDDK-tag pAb

Code No.　PM020

Anti-DDDDK-tag pAb

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 18, 2024 in JST）

Size

100 µL

Data

  • Western Blotting

  • Immunoprecipitation

  • Immunocytochemistry
Clonality Polyclonal Clone Polyclonal
Isotype (Immunized Animal) Rabbit Ig (aff.)
Applications
WB
1:1,000  
IP
5 µL/sample  
IC
1:1,000  
IH*
reproted.  （PMID: 30206360）
IF*
reported.  
Immunogen (Antigen) KLH-conjugated synthetic peptide, DYKDDDDK
Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Background Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. Short sequences encoding the epitope tag are cloned in-frame with target DNA to produce fusion proteins containing the epitope tag peptide. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Anti-epitope tag antibodies can serve as universal purification or detection reagents for any tag-containing protein. The DDDDK epitope tag peptide sequence (DYKDDDDK) was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. The DDDDK peptide has been widely used as a multi-purpose tag, and anti-DDDDK antibodies are optimally suited for identifying, detecting, purifying, and monitoring the expression levels of recombinant DDDDK fusion proteins.
Related products PM020-7 Anti-DDDDK-tag pAb-HRP-DirecT
PM020-8 Anti-DDDDK-tag pAb-Agarose
M185-3L Anti-DDDDK-tag mAb
M185-7 Anti-DDDDK-tag mAb-HRP-DirecT
M185-A48 Anti-DDDDK-tag mAb-Alexa Fluor™ 488
M185-A59 Anti-DDDDK-tag mAb-Alexa Fluor™ 594
M185-A64 Anti-DDDDK-tag mAb-Alexa Fluor™ 647
M185-6 Anti-DDDDK-tag mAb-Biotin
Citations

Western Blotting

  1. Takahashi S et al. The E3 ubiquitin ligase LNX1p80 promotes the removal of claudins from tight junctions in MDCK cells. J Cell Sci. 122, 985-94 (2009)(PMID:19295125)
  2. Inageda K. Insulin modulates induction of glucose-regulated protein 78 during endoplasmic reticulum stress via augmentation of ATF4 expression in human neuroblastoma cells. FEBS Lett. 584, 3649-54 (2010)(PMID:20667453)
  3. Itsumura N et al. Compound Heterozygous Mutations in SLC30A2/ZnT2 Results in Low Milk Zinc Concentrations: A Novel Mechanism for Zinc Deficiency in a Breast-Fed Infant. PLoS One. 8, e64045 (2013)(PMID:23741301)
  4. Kohno T et al. Importance of Reelin C-terminal region in the development and maintenance of the postnatal cerebral cortex and its regulation by specific proteolysis. J Neurosci. 35, 4776-87 (2015)(PMID:25788693)
  5. Matsuo E et al. Development of reverse genetics for Ibaraki virus to produce viable VP6-tagged IBAV. FEBS Open Bio. 5, 445-53 (2015)(PMID:26101741)
  6. Makise M et al. The role of vimentin in the tumor marker Nup88-dependent multinucleated phenotype. BMC Cancer 18, 519 (2018)(PMID:29724197)
  7. Watanabe S et al. MDC1 methylation mediated by lysine methyltransferases EHMT1 and EHMT2 regulates active ATM accumulation flanking DNA damage sites. Sci Rep. 8, 10888 (2018)(PMID:30022091)
  8. Baba K et al. Gradient-reading and mechano-effector machinery for netrin-1-induced axon guidance. Elife 7, e34593 (2018)(PMID:30082022)
  9. Chen Y et al. PHLDA1, another PHLDA family protein that inhibits Akt. Cancer Sci. 109, 3532-3542 (2018)(PMID:30207029)
  10. Murata H et al. c-Jun N-terminal kinase (JNK)-mediated phosphorylation of SARM1 regulates NAD+ cleavage activity to inhibit mitochondrial respiration. J Biol Chem. 293, 18933-18943 (2018)(PMID:30333228)
  11. Zheng CL et al. Hepatitis B virus core protein dimer‑dimer interface is critical for viral replication. Mol Med Rep. 19, 262-270 (2019) (PMID:30387827)
  12. Gao Q et al. A Novel CNGA1 Gene Mutation (c.G622A) of Autosomal Recessive Retinitis Pigmentosa Leads to the CNGA1 Protein Reduction on Membrane. Biochem Genet. in press (PMID:30652268)
  13. Pan T et al. USP49 potently stabilizes APOBEC3G protein by removing ubiquitin and inhibits HIV-1 replication. Elife. 8, e48318 (2019)(PMID:31397674)
  14. Koyano F et al. Parkin-mediated ubiquitylation redistributes MITOL/March5 from mitochondria to peroxisomes. EMBO Rep. 20, e47728 (2019)(PMID:31602805)
  15. Yamano S et al. Nuclear import of IER5 is mediated by a classical bipartite nuclear localization signal and is required for HSF1 full activation. Exp Cell Res. 386, 111686 (2019)(PMID:31669744)
  16. Hua R et al. FBXO47 regulates telomere-inner nuclear envelope integration by stabilizing TRF2 during meiosis. Nucleic Acids Res. 47, 11755-11770 (2019) (PMID:31724724)
  17. Kobayashi S et al. West Nile Virus Capsid Protein Inhibits Autophagy by AMP-activated Protein Kinase Degradation in Neurological Disease Development. PLoS Pathog. 16, e1008238 (2020)(PMID:31971978)
  18. Liu C et al. Slx5p-Slx8p Promotes Accurate Chromosome Segregation by Mediating the Degradation of Synaptonemal Complex Components During Meiosis. Adv Sci (Weinh). 7, 1900739 (2020)(PMID:32099749)
  19. Miyata H et al. Testis-enriched kinesin KIF9 is important for progressive motility in mouse spermatozoa. FASEB J. 34, 5389-5400 (2020)(PMID:32072696)
  20. Yamamoto YH et al. ERdj8 governs the size of autophagosomes during the formation process. J Cell Biol. 219, e201903127 (2020)(PMID:32492081)
  21. Yamaguchi H et al. Wipi3 is essential for alternative autophagy and its loss causes neurodegeneration. Nat Commun. 11, 5311 (2020) (PMID:33082312)
  22. Fukuda T et al. Atg43 tethers isolation membranes to mitochondria to promote starvation-induced mitophagy in fission yeast. Elife. 9, e61245 (2020)(PMID:33138913)

Co-IP

  1. Watanabe S et al. MDC1 methylation mediated by lysine methyltransferases EHMT1 and EHMT2 regulates active ATM accumulation flanking DNA damage sites. Sci Rep. 8, 10888 (2018)(PMID:30022091)

Immunoprecipitation

  1. Sugiyama T et al. Red5 and three nuclear pore components are essential for efficient suppression of specific mRNAs during vegetative growth of fission yeast. Nucleic Acids Res. 41, 6674-86 (2013)(PMID:23658229)
  2. Liu Y et al. Identification of Novel MAGE-G1-Interacting Partners in Retinoic Acid-Induced P19 Neuronal Differentiation Using SILAC-Based Proteomics. Sci Rep. 7, 44699 (2017)(PMID:28374796)
  3. An Y et al. The nuclear GSK-3β regulated post-transcriptional processing of mRNA through phosphorylation of SC35. Mol Cell Biochem. (2018) In press.(PMID:30030778)
  4. Pan T et al. USP49 potently stabilizes APOBEC3G protein by removing ubiquitin and inhibits HIV-1 replication. Elife. 8, e48318 (2019)(PMID:31397674)
  5. Yamano S et al. Nuclear import of IER5 is mediated by a classical bipartite nuclear localization signal and is required for HSF1 full activation. Exp Cell Res. 386, 111686 (2019)(PMID:31669744)
  6. Kobayashi S et al. West Nile Virus Capsid Protein Inhibits Autophagy by AMP-activated Protein Kinase Degradation in Neurological Disease Development. PLoS Pathog. 16, e1008238 (2020)(PMID:31971978)

Immunocytochemistry

  1. Fukunaka A et al. Tissue nonspecific alkaline phosphatase is activated via a two-step mechanism by zinc transport complexes in the early secretory pathway. J Biol Chem. 286, 16363-73 (2011)(PMID:21402707)
  2. Kurio H et al. Identification of CEACAM6 as an intermediate filament-associated protein expressed in Sertoli cells of rat testis. Biol Reprod. 85, 924-33 (2011)(PMID:21734260)
  3. Kitagawa M et al. Targeting Aurora B to the equatorial cortex by MKlp2 is required for cytokinesis. PLoS One 8, e64826 (2013)(PMID:23750214)
  4. Murata H et al. SARM1 and TRAF6 bind to and stabilize PINK1 on depolarized mitochondria. Mol Biol Cell 24, 2772-84 (2013)(PMID:23885119)
  5. Matsuo E et al. Development of reverse genetics for Ibaraki virus to produce viable VP6-tagged IBAV. FEBS Open Bio. 5, 445-53 (2015)(PMID:26101741)
  6. Iimori M et al. Phosphorylation of EB2 by Aurora B and CDK1 ensures mitotic progression and genome stability. Nat Commun. 7, 11117 (2016)(PMID:27030108)
  7. Ishiyama N et al. Force-dependent allostery of the α-catenin actin-binding domain controls adherens junction dynamics and functions. Nat Commun. 9, 5121 (2018)(PMID:30504777)
  8. Gao Q et al. A Novel CNGA1 Gene Mutation (c.G622A) of Autosomal Recessive Retinitis Pigmentosa Leads to the CNGA1 Protein Reduction on Membrane. Biochem Genet. in press(PMID:30652268)
  9. Yamano S et al. Nuclear import of IER5 is mediated by a classical bipartite nuclear localization signal and is required for HSF1 full activation. Exp Cell Res. 386, 111686 (2019)(PMID:31669744)
  10. Kobayashi S et al. West Nile Virus Capsid Protein Inhibits Autophagy by AMP-activated Protein Kinase Degradation in Neurological Disease Development. PLoS Pathog. 16, e1008238 (2020)(PMID:31971978)
  11. Yamamoto YH et al. ERdj8 governs the size of autophagosomes during the formation process. J Cell Biol. 219, e201903127 (2020)(PMID:32492081)

Immunohistochemistry

  1. Li J et al. Differential effects of spinal motor neuron-derived and skeletal muscle-derived Rspo2 on acetylcholine receptor clustering at the neuromuscular junction. Sci Rep. 8, 13577 (2018)(PMID:30206360)
  2. Koyano F et al. Parkin-mediated ubiquitylation redistributes MITOL/March5 from mitochondria to peroxisomes. EMBO Rep. 20, e47728 (2019)(PMID:31602805)

Immunofluorescence

  1. Yamaguchi H et al. Wipi3 is essential for alternative autophagy and its loss causes neurodegeneration. Nat Commun. 11, 5311 (2020) (PMID:33082312)
Product category
Tools
Epitope tags
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  • The availability is based on the information in Japan at 17:00, Apr 18, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.