Anti-V5-tag pAb-Agarose

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 20, 2024 in JST）

Size

Gel:200 µL (Slurry:400 µL)

Citations

Data Sheet

SDS

Data	Immunoprecipitation
Clonality	Polyclonal	Clone	Polyclonal
Isotype (Immunized Animal)	Rabbit Ig (aff.)
Applications	IP 20 µL of gel slurry
Immunogen (Antigen)	Carrier protein conjugated synthetic peptide (CP-GKPIPNPLLGLDST)
Storage buffer	400 µg of anti-V5-tag polyclonal antibody covalently coupled to 200 µL of agarose gel and provided as a 50% gel slurry (in PBS/0.09% NaN₃)
Storage temp.	4°C	Conjugate	Agarose	Manufacturer	MBL
Background	Expression vectors containing a protein and a tag peptide are commonly used. V5-tag fusion protein expression system is preferably used in various laboratories. This specific antibody for V5-tag fusion protein is a useful tool for monitoring of the fusion protein expression and affinity purification.
Related products	PM003 Anti-V5-tag pAb M167-3 Anti-V5-tag mAb M167-10 Anti-V5-tag mAb-Magnetic Agarose 3315-205 V5-tag peptide M215-7 Anti-V5-tag mAb-HRP-DirecT M215-6 Anti-V5-tag mAb-Biotin 3318 V5-tagged Protein Purification Gel Ver.2 3317A V5-tagged Protein Purification Kit Ver.2 (Trial Kit) 3317 V5-tagged Protein Purification Kit Ver.2 3341 V5-tagged Protein Magnetic Purification Kit M167-11 Anti-V5-tag mAb-Magnetic Beads M215-3 Anti-V5-tag mAb M217-3 Anti-Phospho-p62 (SQSTM1) (Ser351) mAb M215-11 Anti-V5-tag mAb-Magnetic Beads
Citations	Co-IP Uenaka T et al. In silico drug screening by using genome-wide association study data repurposed dabrafenib, an anti-melanoma drug, for Parkinson's disease. Hum Mol Genet. 27, 3974-3985 (2018)(PMID:30137437) Wu X et al. JOSD1 inhibits mitochondrial apoptotic signalling to drive acquired chemoresistance in gynaecological cancer by stabilizing MCL1. Cell Death Differ. In press(PMID:31043700) Taira J et al. Dephosphorylation of clustered phosphoserine residues in human Grb14 by protein phosphatase 1 and its effect on insulin receptor complex formation. J Pept Sci. 25, e3207 (2019)(PMID:31347216)
Product category	Tools Epitope tags

The availability is based on the information in Japan at 17:00, Apr 20, 2024 in JST.
The special price is shown in red color.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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