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HOME > Product search results > Code No. PD048 Anti-OsTIR1 pAb

Code No. PD048

Anti-OsTIR1 pAb

Availability (in Japan)

10 or more

(In Japan at 00:05,
Nov 21, 2018 in JST)

Size

100 µL

Data
  • Western Blotting

Clonality Polyclonal
Isotype (Immunized Animal) Rabbit IgG
Applications
WB
1:1,000  
Immunogen (Antigen) Recombinant full-length Oryza sativa Transport inhibitor response 1 (OsTIR1) protein
Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names Oryza sativa Transport inhibitor response 1
Background The auxin-inducible degron (AID) system allows the rapid and reversible proteolysis of proteins of interest, which have been invented by Dr. Masato Kanemaki in National Institute of Genetics in Japan. OsTIR1 is a F-box family protein specifically expressed in a rice plant. Since OsTIR1 can function under a culture condition at 37 degree, it is integrated in the AID system to work it in mammalian cells. Briefly, OsTIR1 binds to endogenous Skp1 to form the Skp1-Cullin1-OsTIR1 complex (SCFTIR1). SCFTIR1 binds and ubiquitinates the AID tag in the presence of auxin, and promotes proteasome-dependent degradation of the AID-tagged target protein. Human HCT116 cell lines harboring OsTIR1 (CMV-OsTIR1 RCB4662 or Tet-OsTIR1) are available from the RIKEN Cell Bank.
Related products M214-3 Anti-mini-AID-tag mAb
M214-7 Anti-mini-AID-tag mAb-HRP-DirecT
References
  1. Natsume T et al. Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors. Cell Rep. 15, 210-8 (2016)(PMID: 27052166
Product category
Research area
Ubiquitin proteasome
Tools
Others
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  • The availability is based on the information in Japan at 00:05, Nov 21, 2018 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
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