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HOME > Product search results > Code No. PD021 Anti-β5t (Mouse) pAb

Code No. PD021

Anti-β5t (Mouse) pAb

Availability (in Japan)

10 or more

(In Japan at 00:05,
Sep 20, 2018 in JST)

Size

100 µL

Clonality Polyclonal Clone Polyclonal
Isotype (Immunized Animal) Rabbit IgG
Applications
WB
1:1,000-1:2,000  
IP
1 µL/50 µg of mouse thymus extract  
IC
1:250-1:500  
IH
1:250-1:500 (frozen section)  
Immunogen (Antigen) Recombinant Mouse β5t (234-302 a.a.)
Reactivity [Gene ID]

Mouse[73902]

Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names Psmb11, beta5t
Background

The 20S proteasome is composed of 28 subunits, arranged as a cylinder-shaped structure made of four stacked hetero-heptameric rings: α1-7, β1-7, β1-7, and α1-7. β1, β2, and β5 function the catalytic subunit for proteolysis. Murata and colleagues recently identified a new catalytic β subunit called β5t, which is only expressed in thymic cortical epithelial cells and dominantly incorporated into thymic 20S proteasomes. The integration of β5t into proteasomes reduces their chymotrypsin-like activity responsible for producing high affinity peptides for MHC class I. The depletion of β5t in mice resulted in defective development of CD8 single positive T cells in the thymus, indicating that β5t has a key role for generating the MHC class I-restricted CD8+ T cell repertoire during thymic selection. There results challenge the 15 years old theory of T-cell development, particularly positive selection in the thymus and the phenomenon of peripheral tolerance.

Citations

Immunohistochemistry

  1. Reeh KA et al. Ectopic TBX1 suppresses thymic epithelial cell differentiation and proliferation during thymus organogenesis. Development 141, 2950-8 (2014)(PMID:25053428)
  2. Žuklys S et al. Foxn1 regulates key target genes essential for T cell development in postnatal thymic epithelial cells. Nat Immunol. 17, 1206-1215 (2016)(PMID:27548434)
  3. Xiao S et al. Thymic B cell development is controlled by the B potential of progenitors via both hematopoietic-intrinsic and thymic microenvironment-intrinsic regulatory mechanisms. PLoS One 13, e0193189 (2018)(PMID:29462202)
Product category
Research area
Immunology
Ubiquitin proteasome
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  • The availability is based on the information in Japan at 00:05, Sep 20, 2018 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.