Anti-CD63 (LAMP-3) mAb

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 19, 2024 in JST）

Size

100 µL (1 mg/mL)

Citations

Data Sheet

SDS

Data	Western Blotting non-reducing condition Immunoprecipitation Immunoprecipitation Immunoprecipitation under evaluation Flow Cytometry Flow Cytometry ELISA under evaluation
Clonality	Monoclonal	Clone	C047-1
Isotype (Immunized Animal)	Mouse IgG2bκ
Applications	WB 1 µg/mL (non-reducing condition) IP 5 µg/sample FCM 5 µg/mL (final concentration) ELISA Can be used.
Immunogen (Antigen)	human prostate carcinoma cell line (PC3) derived exosomes (prepared by ultracentrifugation from cultured supernatant)
Reactivity [Gene ID]	Human[967], Monkey[103238483]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	MIC3, MRP-1, BTCC-1, DRAP-27, TSPAN29, TSPAN-29
Background	CD63 antigen, also known as LAMP-3, is a tetraspanin characterized by four transmembrane domains and a member of the transmembrane 4 superfamily. CD63 is the first characterized tetraspanin. CD63 has broad tissue distribution and may play multiple roles in a variety of cellular processes such as immune response and tumorigenesis, but its function has not been elucidated entirely. It is reported that CD63 is abundant in tetraspanin-enriched microdomains (TEMs) and a certain type of cellular compartment, such as late endosomes and lysosomes, while the tetraspanin-rich small lipid bilayers are secreted into extracellular fluid as a certain type of extracellular vesicles, called exosome. Because of various exosome features, CD63, one of the representative exosome surface proteins, is thought to be related to intercellular communication.
Related products	D263-3 Anti-CD63 (LAMP-3) (Mouse) mAb M076-3 Mouse IgG2a (isotype control) M077-3 Mouse IgG2b (isotype control) MEX002-3 Anti-CD63 (LAMP-3) mAb MEX003-3 Anti-CD81 (TAPA1) mAb 3190 Magnetic Rack MJS002V2 Protein G-Magnetic Beads MEX001-6 Anti-CD9 mAb-Biotin MEX002-6 Anti-CD63 (LAMP-3) mAb-Biotin MEX003-6 Anti-CD81 (TAPA1) mAb-Biotin MEX001-3 Anti-CD9 mAb MEX001-4 Anti-CD9 mAb-FITC MEX002-4 Anti-CD63 (LAMP-3) mAb-FITC MEX003-4 Anti-CD81 (TAPA1) mAb-FITC MEX001-12 Anti-CD9 mAb-ALP MEX002-12 Anti-CD63 (LAMP-3) mAb-ALP MEX003-12 Anti-CD81 (TAPA1) mAb-ALP
Citations	Western Blotting Men Y et al. Exosome reporter mice reveal the involvement of exosomes in mediating neuron to astroglia communication in the CNS. Nat Commun. 10, 1038 (2019)(PMID:31515491) Ohzawa H et al. Exosomal microRNA in peritoneal fluid as a biomarker of peritoneal metastases from gastric cancer. Ann Gastroenterol Surg. 4, 84-93 (2019) (PMID:32021962) Ohzawa H et al. Reduced expression of exosomal miR‑29s in peritoneal fluid is a useful predictor of peritoneal recurrence after curative resection of gastric cancer with serosal involvement. Oncol Rep. 43, 1081-1088 (2020)(PMID:32323853)
References	Mitsunari T et al. Clathrin adaptor AP-2 is essential for early embryonal development. Mol Cell Biol. 25, 9318-23 (2005)（PMID: 16227583） Nishida K et al. Fc{epsilon}RI-mediated mast cell degranulation requires calcium-independent microtubule-dependent translocation of granules to the plasma membrane. J Cell Biol. 170, 115-26 (2005)（PMID: 15998803） Kabu K et al. Zinc is required for Fc epsilon RI-mediated mast cell activation. J Immunol. 177, 1296-1305 (2006)（PMID: 16818790） Liu Y et al. An essential role for RasGRP1 in mast cell function and IgE-mediated allergic response. J Exp Med. 204, 93-103 (2007)（PMID: 17190838） Simons M, Raposo G. Exosomes--vesicular carriers for intercellular communication. Curr Opin Cell Biol. 21, 575-81 (2009)（PMID: 19442504） Pols MS, Klumperman J. Trafficking and function of the tetraspanin CD63. Exp Cell Res. 315, 1584-92 (2009)（PMID: 18930046） Yoshioka Y et al. Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen. Nat Commun. 5, 3591 (2014)（PMID: 24710016） Melo SA et al. Glypican-1 identifies cancer exosomes and detects early pancreatic cancer. Nature 523, 177-82 (2015)（PMID: 26106858）
Product category	Research area Immunology RNA-RNP Network Cancer Cell surface antigens Exosomes Brands ExoCap™

The availability is based on the information in Japan at 17:00, Apr 19, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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