Anti-CD9 mAb

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 16, 2024 in JST）

Size

100 µL (1 mg/mL)

Citations

Data Sheet

SDS

Data	Western Blotting non-reducing condition Immunoprecipitation Immunoprecipitation Immunoprecipitation Flow Cytometry Flow Cytometry ELISA ELISA
Clonality	Monoclonal	Clone	A100-4
Isotype (Immunized Animal)	Mouse IgG2aκ
Applications	WB 1 µg/mL (non-reducing condition) IP 5 µg/sample FCM 5 µg/mL (final concentration) ELISA Can be used. Other(Immunocaputre of exosome) reported. （PMID: 28851650）
Immunogen (Antigen)	human prostate carcinoma cell line (PC3) derived exosomes (prepared by ultracentrifugation from cultured supernatant)
Reactivity [Gene ID]	Human[928], Mouse(-), Rat(-), Hamster(-), Monkey[103218443]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	5H9, BA2, BTCC-1, DRAP-27, GIG2, MIC3, MRP-1, P24, TSPAN29
Background	CD9, a member of the tetraspanin or transmembrane 4 super family (TM4SF; CD37, CD53, CD63, CD81, CD82, etc) of proteins, is widely distributed on the surface of a variety of hematopoietic and epithelial cell types. Several members of the tetraspanin family, including CD9, form noncovalent associations with integrins, particularly β1 integrins. CD9 and other tetraspanins have been postulated to participate in the regulation of cell growth, motility, and signaling via their physical association with integrins. CD9 has broad tissue distribution and may play multiple roles in a variety of cellular processes such as immune response and tumorigenesis, but its function has not been elucidated entirely. It is reported that the tetraspanins CD9, CD63 and CD81 are highly enriched on a certain type of extracellular vesicles, called exosome. Because of various exosome features, CD9, one of the representative exosome surface proteins, is thought to be related to cell-cell communication.
Related products	D263-3 Anti-CD63 (LAMP-3) (Mouse) mAb M076-3 Mouse IgG2a (isotype control) M077-3 Mouse IgG2b (isotype control) MEX002-3 Anti-CD63 (LAMP-3) mAb MEX003-3 Anti-CD81 (TAPA1) mAb 3190 Magnetic Rack MJS002V2 Protein G-Magnetic Beads MEX001-6 Anti-CD9 mAb-Biotin MEx002-6 Anti-CD63 (LAMP-3) mAb-Biotin MEX003-6 Anti-CD81 (TAPA1) mAb-Biotin MEX001-4 Anti-CD9 mAb-FITC MEX002-4 Anti-CD63 (LAMP-3) mAb-FITC MEX003-4 Anti-CD81 (TAPA1) mAb-FITC MEX001-12 Anti-CD9 mAb-ALP MEX002-12 Anti-CD63 (LAMP-3) mAb-ALP MEX003-12 Anti-CD81 (TAPA1) mAb-ALP
Citations	Other(Immunocaputre of exosome) Horie K et al. Exosomes expressing carbonic anhydrase 9 promote angiogenesis. Biochem Biophys Res Commun. 492, 56-361 (2017)(PMID:28851650)
References	Boucheix C et al. Molecular cloning of the CD9 antigen. A new family of cell surface proteins. J Biol Chem. 266, 117-22 (1991)（PMID: 1840589） Simons M, Raposo G. Exosomes--vesicular carriers for intercellular communication. Curr Opin Cell Biol. 21, 575-81 (2009)（PMID: 19442504） Pols MS, Klumperman J. Trafficking and function of the tetraspanin CD63. Exp Cell Res. 315, 1584-92 (2009)（PMID: 18930046） Yoshioka Y et al. Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen. Nat Commun. 5, 3591 (2014)（PMID: 24710016） Melo SA et al. Glypican-1 identifies cancer exosomes and detects early pancreatic cancer. Nature 523, 177-82 (2015)（PMID: 26106858）
Product category	Research area RNA-RNP Network Cancer Cell surface antigens Exosomes Brands ExoCap™

The availability is based on the information in Japan at 17:00, Apr 16, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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