This site is for customers in Asia. Customers in China & other regions, please go to Global page.
HOME > Product search results > Code No. M171-3 Anti-GAPDH mAb

Code No. M171-3

Anti-GAPDH mAb

Availability (in Japan)

10 or more

(In Japan at 00:05,
Apr 19, 2024 in JST)

Size

100 µL (3 mg/mL)

Data
  • Western Blotting

Clonality Monoclonal Clone 3H12
Isotype (Immunized Animal) Mouse IgG2aκ
Applications
WB
3 µg/mL  
Immunogen (Antigen) GAPDH from Rabbit muscle
Reactivity [Gene ID]

Human[2597], Mouse[14433], Rat[24383], Hamster[100736557], Chicken[374193], Monkey*(PMID: 29026117)

Storage buffer 3 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD
Background GAPDH (Glyceraldehyde- 3-phosphate Dehydrogenase) is a well-known enzyme, which catalyzes of glycolysis. GAPDH is stably and constitutively expressed at high levels in most tissues and cells, it is considered a housekeeping protein. Therefore, GAPDH is often used as a loading control of WB.
Related products M171-7 Anti-GAPDH mAb-HRP-DirecT
PM088 Anti-Vinculin pAb
M175-3 Anti-α-Tubulin mAb
M177-3 Anti-β-Actin mAb
Citations

Western Blotting

  1. Hasegawa M et al. ASC-mediated NF-kappaB activation leading to interleukin-8 production requires caspase-8 and is inhibited by CLARP. J Biol Chem. 280, 15122-30 (2005)(PMID:15701651)
  2. Li Q et al. The cleavage pattern of TDP-43 determines its rate of clearance and cytotoxicity. Nat Commun. 6, 6183 (2015)(PMID:25630387)
  3. Liu S et al. Increased glyceraldehyde-3-phosphate dehydrogenase expression indicates higher survival rates in male patients with hepatitis B virus-accociated hepatocellular carcinoma and cirrhosis. Exp Ther Med. 9, 1597-1604 (2015)(PMID:26136865)
  4. Mita T et al. Docosahexaenoic Acid Promotes Axon Outgrowth by Translational Regulation of Tau and Collapsin Response Mediator Protein 2 Expression. J Biol Chem. 291, 4955-65 (2016)(PMID:26763232)
  5. Yoshimoto R et al. Global analysis of pre-mRNA subcellular localization following splicing inhibition by spliceostatin A. RNA 23, 47-57 (2017)(PMID:27754875)
  6. Miyake K et al. CAPS1 RNA Editing Promotes Dense Core Vesicle Exocytosis. Cell Rep. 17, 2004-2014 (2016)(PMID:27851964)
  7. Gao Y et al. Comprehensive proteome analysis of lysosomes reveals the diverse function of macrophages in immune responses. Oncotarget 8, 7420-7440 (2017)(PMID:28088779)
  8. FuadHafid A et al. Antiviral activity of the dichloromethane extracts from Artocarpus heterophyllus leaves against hepatitis C virus. Asian Pac J Trop Biomed. 7, 633-639 (2017)
  9. Uemura Y et al. Matrin3 binds directly to intronic pyrimidine-rich sequences and controls alternative splicing. Genes Cells. 22, 785-798 (2017)(PMID:28695676)
  10. Xu J et al. Nuclear carbonic anhydrase 6B associates with PRMT5 to epigenetically promote IL-12 expression in innate response. PNAS. 114, 8620-8625 (2017)(PMID:28739930)
  11. Lv J et al. PCDH9 acts as a tumor suppressor inducing tumor cell arrest at G0/G1 phase and is frequently methylated in hepatocellular carcinoma. Mol Med Rep. 16, 4475-4482 (2017)(PMID:28791409)
  12. Hwang SY et al. Synthesis and biological effect of chrom-4-one derivatives as functional inhibitors of heat shock protein 27. Eur J Med Chem. 139, 892-900 (2017)(PMID:28869891)
  13. Wang S et al. Inhibition of the deubiquitinase USP5 leads to c-Maf protein degradation and myeloma cell apoptosis. Cell Death Dis. 8, e3058 (2017)(PMID:28933784)
  14. Li L et al. ZNF516 suppresses EGFR by targeting the CtBP/LSD1/CoREST complex to chromatin. Nat Commun. 8, 691 (2017)(PMID:28947780)
  15. Chen L et al. MicroRNA-199a acts as a potential suppressor of cardiomyocyte autophagy through targeting Hspa5. Oncotarget 8, 63825-63834 (2017)(PMID:28969032)
  16. Miyamoto Y et al. Striatal N-Acetylaspartate Synthetase Shati/Nat8l Regulates Depression-Like Behaviors via mGluR3-Mediated Serotonergic Suppression in Mice. Int J Neuropsychopharmacol. 20, 1027-1035 (2017)(PMID:29020418)
  17. Fu K et al. Involvement of the accumbal osteopontin-interacting transmembrane protein 168 in methamphetamine-induced place preference and hyperlocomotion in mice. Sci Rep. 7, 13084 (2017)(PMID:29026117)
  18. Ju J et al. NatD promotes lung cancer progression by preventing histone H4 serine phosphorylation to activate Slug expression. Nat Commun. 8, 928 (2017)(PMID:29030587)
  19. Ichikawa H et al. Overexpression of exchange protein directly activated by cAMP-1 (EPAC1) attenuates bladder cancer cell migration. Biochem Biophys Res Commun. 495, 64-70 (2018)(PMID:29111327)
  20. Huang Y et al. RLIM suppresses hepatocellular carcinogenesis by up-regulating p15 and p21. Oncotarget. 8, 83075-83087 (2017)(PMID:29137325)
  21. Mori T et al. Structural basis of thalidomide enantiomer binding to cereblon. Sci Rep. 8, 1294 (2018)(PMID:29358579)
  22. Shinto S et al. Antioxidant nitroxides protect hepatic cells from oxidative stress-induced cell death. J Clin Biochem Nutr. 62, 132-138 (2018)(PMID:29610552)
  23. Wang D et al. MiR-27a Promotes Hemin-Induced Erythroid Differentiation of K562 Cells by Targeting CDC25B. Cell Physiol Biochem. 46, 365-374 (2018)(PMID:29590650)
  24. Mizuta R et al. Cow's milk neutralizes the cytotoxicity of acrolein, a putative carcinogen in cigarette smoke. J Vet Med Sci. 80, 1301-1304 (2018)(PMID:29937461)
  25. Sakane H, Akasaki K. The Major Lysosomal Membrane Proteins LAMP-1 and LAMP-2 Participate in Differentiation of C2C12 Myoblasts. Biol Pharm Bull. 41, 1186-1193 (2018)(PMID:30068868)
  26. Oh-Hashi K et al. Expression analysis and functional characterization of the mouse cysteine-rich with EGF-like domains 2. Sci Rep. 8, 12236 (2018)(PMID:30111858)
  27. Nakahara M et al. Effect of a p38 Mitogen-Activated Protein Kinase Inhibitor on Corneal Endothelial Cell Proliferation. Invest Ophthalmol Vis Sci. 59, 4218-4227 (2018)(PMID:30128493)
  28. Fujibayashi E et al. Isolation of cancer cells with augmented spheroid-forming capability using a novel tool equipped with removable filter. Oncotarget 9, 33931-33946 (2018)(PMID:30338036)
  29. Sun D et al. Polycomb chromobox Cbx2 enhances antiviral innate immunity by promoting Jmjd3-mediated demethylation of H3K27 at the Ifnb promoter. Protein Cell (2018) In press.(PMID:30357595)
  30. Wang C et al. S100A16 regulated by Snail promotes the chemoresistance of nonmuscle invasive bladder cancer through the AKT/Bcl-2 pathway. Cancer Manag Res. 11, 2449-2456 (2019) (PMID:31118765)
  31. Tsuchiya K <em>et al. Caspase-1 initiates apoptosis in the absence of gasdermin D. Nat Commun. 10, 2091 (2019)(PMID:31064994)
  32. Yin G et al. miR 221 3p promotes the cell growth of non small cell lung cancer by targeting p27. Mol Med Rep. 20, 604-612 (2019)(PMID:31180541)
  33. Sanjo H et al. Cutting Edge: TAK1 Safeguards Macrophages against Proinflammatory Cell Death. J Immunol. 203, 783-788 (2019)(PMID:31243089)
  34. Araki K et al. TDP-43 regulates early-phase insulin secretion via CaV1.2-mediated exocytosis in islets. J Clin Invest. 130, 3578-3593 (2019)(PMID:31355778)
  35. Huang JH et al. Arginine vasopressin modulates electrical activity and calcium homeostasis in pulmonary vein cardiomyocytes. J Biomed Sci. 26, 1186 (2019) (PMID:31530276)
  36. Kamohara A et al. IgG immune complexes with Staphylococcus aureus protein A enhance osteoclast differentiation and bone resorption by stimulating Fc receptors and TLR2. Int Immunol. 32, 89-104 (2019)(PMID:31713625)
  37. Morinaga A et al. RBM20 Regulates CaV1.2 Surface Expression by Promoting Exon 9* Inclusion of CACNA1C in Neonatal Rat Cardiomyocytes. Int J Mol Sci. 20, pii: E5591 (2019)(PMID:31717392)
  38. Fujimoto N et al. Morphological and functional changes in neonatally X-irradiated thyroid gland in rats. Endocr J. 67, 231-240 (2019) (PMID:31748432)
  39. Matsushima S et al. Anosmin-1 Activates Vascular Endothelial Growth Factor Receptor and Its Related Signaling Pathway for Olfactory Bulb Angiogenesis. Sci Rep. 10, 188(PMID:31932617)
  40. Hamada Y et al. Nanosecond pulsed electric fields induce the integrated stress response via reactive oxygen species-mediated heme-regulated inhibitor (HRI) activation. PLoS One. 15, e0229948 (2020)(PMID:32155190)
  41. Tsutsui T et al. Glioma-derived extracellular vesicles promote tumor progression by conveying WT1. Carcinogenesis. 41, 1238-1245 (2020)(PMID:32463428)
  42. Wang Y et al. UBE2C mRNA expression controlled by miR-300 and HuR determines its oncogenic role in gastric cancer. Biochem Biophys Res Commun. 534, 597-603 (2021)(PMID:33223052)
Product category
Tools
Control antibodies
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.