Anti-MBP (Maltose Binding Protein) mAb

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 17, 2024 in JST）

Size

100 µL (1 mg/mL)

Citations

Data Sheet

SDS

Data	Immunoprecipitation
Clonality	Monoclonal	Clone	1G12
Isotype (Immunized Animal)	Mouse IgG3
Applications	WB 1 µg/mL IP 2 µg/sample
Immunogen (Antigen)	Recombinant MBP
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Background	Maltose Binding Protein (MBP) is a part of the maltose/maltodextrin system of Eschericia coli. bacteria, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes. MBP is often used as a fusion partner for recombinant protein expression, primarily because its affinity for maltodextrins allows for facile purification of fusion proteins by amylose affinity chromatography.
Citations	Western Blotting Ito T et al. Alteration of substrate specificity: the variable N-terminal domain of tobacco Ca(2+)-dependent protein kinase is important for substrate recognition. Plant Cell 22, 1592-604 (2010)(PMID:20442373) Kasahara K et al. EGF receptor kinase suppresses ciliogenesis through activation of USP8 deubiquitinase. Nat Commun. 9, 758 (2018)(PMID:29472535) Ito T et al. Autophosphorylation of Ser-6 via an intermolecular mechanism is important for the rapid reduction of NtCDPK1 kinase activity for substrate RSG. PLoS One 13, e0196357 (2018)(PMID:29684069) Shu T et al. Ebola virus VP35 has novel NTPase and helicase-like activities. Nucleic Acids Res. 47, 5837-5851 (2019)(PMID:31066445) Immunoprecipitation Kasahara K et al. Ubiquitin-proteasome system controls ciliogenesis at the initial step of axoneme extension. Nat Commun. 5, 5081 (2014)(PMID:25270598)
Product category	Tools Epitope tags

The availability is based on the information in Japan at 00:05, Apr 17, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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