Anti-Myc-tag mAb-Alexa Fluor™ 488

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 18, 2024 in JST）

Size

100 µL (1 mg/mL)

Citations

Data Sheet

SDS

Data	Flow Cytometry Immunocytochemistry
Clonality	Monoclonal	Clone	PL14
Isotype (Immunized Animal)	Mouse IgG1 κ
Applications	FCM 2-5 µg/mL (final concentration) IC 5 µg/mL
Immunogen (Antigen)	6myc-tag fusion protein
Storage buffer	PBS containing 1% BSA and 0.1% ProClin 150
Storage temp.	4°C	Conjugate	Alexa Fluor® 488	Manufacturer	MBL
Background	Epitope tagging has widely been accepted technique that fuses an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation and immunofluorescence utilizing with an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follow; YPYDVPDYA (HA-tag), EQKLISEEDL (Myc-tag) and YTDIEMNRLGK (VSV-G-tag), which corresponding to the partial peptide of Influenza hemagglutinin protein, human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.
Related products	M047-3 Anti-Myc-tag mAb M047-8 Anti-Myc-tag mAb-Agarose M047-10 Anti-Myc-tag mAb-Magnetic Agarose M047-A59 Anti-Myc-tag mAb-Alexa Fluor™ 594 M047-A64 Anti-Myc-tag mAb-Alexa Fluor™ 647 M192-3 Anti-Myc-tag mAb M192-7 Anti-Myc-tag mAb-HRP-DirecT 562 Anti-Myc-tag pAb 562-5 Anti-Myc-tag pAb M047-11 Anti-Myc-tag mAb-Magnetic Beads
Citations	Flow Cytometry Rushworth D et al. Dihydrofolate Reductase and Thymidylate Synthase Transgenes Resistant to Methotrexate Interact to Permit Novel Transgene Regulation. J Biol Chem. 290, 22970-6 (2015)(PMID:26242737) Honjoh C et al. Association of C-Type Lectin Mincle with FcεRIβγ Subunits Leads to Functional Activation of RBL-2H3 Cells through Syk. Sci Rep. 7, 46064 (2017)(PMID:28393919) Immunocytochemistry Sangawa T et al. A multipurpose fusion tag derived from an unstructured and hyperacidic region of the amyloid precursor protein. Protein Sci. 22, 840-50 (2013)(PMID:23526492) Ariumi Y et al. DNA repair protein Rad18 restricts LINE-1 mobility. Sci Rep. 8, 15894 (2018)(PMID:30367120) Asanomi Y et al. A rare functional variant of SHARPIN attenuates the inflammatory response and associates with increased risk of late-onset Alzheimer's disease. Mol Med. 25, 20 (2019)(PMID:31216982)
Product category	Tools Epitope tags

The availability is based on the information in Japan at 00:05, Apr 18, 2024 in JST.
The special price is shown in red color.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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