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HOME > Product search results > Code No. D366-3 Anti-SaCas9 mAb

Code No. D366-3

Anti-SaCas9 mAb

Availability (in Japan)

10 or more

(In Japan at 01:30,
Dec 15, 2017 in JST)

Size

100 µg/100 µL

Data
  • Western Blotting

  • Immunoprecipitation

  • Immunocytochemistry

Clonality Monoclonal Clone 11E7-9
Isotype (Immunized Animal) Mouse IgG2aκ
Applications
WB
0.5 µg/mL  
IP
1 μg/sample  
IC
1 µg/mL  
Reactivity [Gene ID]

Other,  Staphylococcus aureus

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names Staphylococcus aureus Cas9, Sa Cas9
Background The CRISPR/Cas9 system is an innovative tool for genome editing. In 2015, a new Cas9 gene was cloned from Staphylococcus aureus. The SaCas9 (Staphylococcus aureus Cas9) gene is 1 kb shorter than the SpCas9 (Streptococcus pyogenes Cas9) gene, which was originally used for genome editing (Ran FA, et al., 2015, Nature). The protospacer adjacent motif (PAM) sequence of SaCas9, required for DNA cleavage, is longer than that of SpCas9. These molecular characteristics of SaCas9 are expected to enable more efficient genome editing.
Related products D366-7 Anti-SaCas9 mAb-HRP-DirecT
Notes Clone 11E7-9 does not cross-react with SpCas9 (Streptococcus pyogenes Cas9).
References
  1. Ran FA et al. In vivo genome editing using Staphylococcus aureus Cas9. Nature 520, 186-91 (2015)(PMID: 27005865
Product category
Tools
Others
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  • The availability is based on the information in Japan at 01:30, Dec 15, 2017 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.