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HOME > Product search results > Code No. D357-3 Anti-Ace (CD143) (Mouse) mAb

Code No. D357-3

Anti-Ace (CD143) (Mouse) mAb

Availability (in Japan)

10 or more

(In Japan at 01:30,
Aug 24, 2017 in JST)

Size

100 µg/100 µL

This antibody (clone 1D5) reacts with both somatic and testis isoforms of Ace (PMID: 16870943). Useful for Western blotting and Immunohistochemistry.
Data
  • Western Blotting

  • Immunohistochemistry

Clonality Monoclonal Clone 1D5
Isotype (Immunized Animal) Mouse IgG2bκ
Applications
WB
1 µg/mL  
IH
1 µg/mL (Heat treatment is necessary for paraffin embedded sections.)  
Immunogen (Antigen) Recombinant protein corresponding to N-terminal region of mouse tACE (testicular ACE) without the signal peptide
Reactivity [Gene ID]

Mouse[11421]

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names angiotensin I converting enzyme (peptidyl-dipeptidase A) 1, DCP, ACE1, DCP1
Background Angiotensin Converting Enzyme(ACE; アンジオテンシン変換酵素)は、Angiotensin I を活性体であるAngiotensin IIに変換することによって血圧を制御する触 媒酵素であり、細胞膜上に存在するタンパク質である。ACEには全身の細胞に広く発現するsomatic ACE(sACE)と、限られた組織にのみ発現するgerminal ACE(gACE)が存在し、gACEは精巣に発現していることが知られている。本モノクローナル抗体(クローン 1D5は、マウス精巣のACE(testicular ACE)を抗原として作製された。Testis-specific isozymeであるtACEは、未成熟精子細胞と成熟した精子の両方に発現していて、受精の際に精子の透明帯結合に関わる分子の一つである。 Calmegin、ADAM1、ADMA2、ADAM3とともにノックアウトすると透明帯に結合できなくなることが知られている。
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PM043 Anti-PIWIL2 (MILI) (Mouse) pAb
PM044 Anti-PIWIL2 (MILI) (Mouse) pAb
D356-3 Anti-Jmjd1c (Mouse) mAb
M077-3 Mouse IgG2b (isotype control)
D362-3 Anti-Tex101 (Mouse) mAb
D363-3 Anti-Ly6k (Mouse) mAb
D365-3 Anti-PIWIL2 (MILI) (Mouse) mAb
References
  1. Hagaman JR et al. Angiotensin-converting enzyme and male fertility. PNAS. 95, 2552-7 (1998)(PMID: 9482924
  2. Yamaguchi R et al. Aberrant distribution of ADAM3 in sperm from both angiotensin-converting enzyme (Ace)- and calmegin (Clgn)-deficient mice. Biol Reprod. 75, 760-6 (2006)(PMID: 16870943
  3. Deguchi E et al. Dipeptidase-inactivated tACE action in vivo: selective inhibition of sperm-zona pellucida binding in the mouse. Biol Reprod. 77, 794-802 (2007)(PMID: 17634445
  4. Fujihara Y et al. Expression of TEX101, regulated by ACE, is essential for the production of fertile mouse spermatozoa. PNAS 110, 8111-6 (2013)(PMID: 23633567
  5. Fujihara Y et al. GPI-anchored protein complex, LY6K/TEX101, is required for sperm migration into the oviduct and male fertility in mice. Biol Reprod. 90, 60 (2014)(PMID: 24501175
Product category
Research area
Stem cell research
Cell surface antigens
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 01:30, Aug 24, 2017 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.