Anti-Mincle (Mouse) mAb-Biotin

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 20, 2024 in JST）

Size

100 µL

Citations

Data Sheet

SDS

Data	Flow Cytometry
Clonality	Monoclonal	Clone	1B6
Isotype (Immunized Animal)	Rat IgG1 κ
Applications	FCM 1:500
Immunogen (Antigen)	RBL-2H3 cells expressing full length mouse mincle
Reactivity [Gene ID]	Mouse[56619]
Storage buffer	PBS/1% BSA/0.1% ProClin 150
Storage temp.	4°C	Conjugate	Biotin	Manufacturer	MBL
Alternative names	Clec4e, C86253, Clecsf9, C-type lectin domain family 4, member e
Background	Macrophage-inducible C-type lectin (Mincle, also called as Clec4e and Clecsf9), a type II transmembrane C-type lectin receptor, is expressed mainly in macrophages. Mincle is selectively associated with the Fc receptor common g-chain which contains immunoreceptor tyrosine-based activation motif (ITAM) and activates macrophages to produce inflammatory cytokines and chemokines. Mincle expressing cells are activated by spliceosome associated protein 130 (SAP130) which is a component of small nuclear ribonucleoprotein released from dead cells. Recently, Mincle is reported to recognize Mycobacterium tuberculosis as well as pathogenic fungus Malassezia species. Mincle is demonstrated to be an essential receptor for a mycobacterial glycolipid, trehalose-6,6’-dimycolate (TDM). The cytokine/chemokine production of macrophages which Malassezia or M.tuberculosis induces in Mincle^-/- mice is significantly impaired. Mincle is considered to play a crucial role in immune responses to pathogens.
Related products	D292-3 Anti-Mincle (Mouse) mAb M191-3 Anti-FcεR1γ (FcRγ) (Mouse) mAb D266-3M2 Anti-Mincle (Mouse) mAb (Functional Grade) D292-3M2 Anti-Mincle (Mouse) mAb (Functional Grade) D360-3 Anti-Mincle (Human) mAb (Functional Grade) D266-3 Anti-Mincle (Mouse) mAb
Citations
References	Miyake Y et al. C-type lectin MCL is an FcRγ-coupled receptor that mediates the adjuvanticity of mycobacterial cord factor. Immunity 38, 1050-62 (2013) Behler F et al. Role of Mincle in alveolar macrophage-dependent innate immunity against mycobacterial infections in mice. J Immunol. 189, 3121-9 (2012) Yamasaki S et al. C-type lectin Mincle is an activating receptor for pathogenic fungus Malassezia. PNAS 106, 1897-1902 (2009) Ishikawa E et al. Direct recognition of the mycobacterial glycolipid, trehalose dimycolate, by C-type lectin Mincle. J Exp Med. 206, 2879-2888 (2009 Yamasaki S et al. Mincle is an ITAM-coupled activating receptor that senses damaged cells. Nat Immunol. 9, 1179-88 (2008)
Product category	Research area Immunology

The availability is based on the information in Japan at 00:05, Apr 20, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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