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HOME > Product search results > Code No. D187-3 Anti-Dlk (Pref-1) mAb

Code No. D187-3

Anti-Dlk (Pref-1) mAb

Availability (in Japan)

1-9

(In Japan at 00:05,
Dec 14, 2018 in JST)

Size

100 µg/100 µL

Data
  • Flow Cytometry

  • Immunohistochemistry

  • Immunohistochemistry

Clonality Monoclonal Clone 24-11
Isotype (Immunized Animal) Rat IgG1
Applications
FCM
5-10 µg/mL  
IC*
reported.  (PMID: 23943621
IH
10 µg/mL (paraffin), 1 µg/mL (frozen)  
Immunogen (Antigen) Pref-1-Fc
Reactivity [Gene ID]

Mouse[13386]

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names delta-like 1 homolog (Drosophila), FA1, ZOG, pG2, DlkI, Peg9, SCP1, DLK-1, Ly107, pref-1, AW742678
Background Delta like protein (Dlk), also known as Preadipocyte factor-1 (Pref-1) or zona glomerulosa-specific factor (ZOG), is an EGF-like transmembrane protein expressed preadipocytes but not in mature adipocytes.  It is highly expressed in fetal liver, the adrenal gland, and placenta, as well as some neuroendocrine tumors and small cell lung carcinomas, where it plays a role in differentiation and proliferation. Dlk positively and negatively regulates adipocyte differentiation via at least four major variants (45-60 kDa) of Dlk generated by alternatively splicing.  Constitutive expression of Dlk inhibits adipogenesis, but insulin or insulin like growth factor-1 (IGF-1) can circumvent this inhibition. Regulated processing of Dlk releases a 50 kDa soluble form that was previously characterized as Fetal Antigen-1, a protein involved in pancreatic island cell differentiation.
Related products D187-4 Anti-Dlk (Pref-1) mAb-FITC
D187-5 Anti-Dlk (Pref-1) mAb-PE
D118-3 Anti-Liv2 (Mouse) mAb
Citations

Flow Cytometry

  1. Khurana S, Mukhopadhyay A. Hematopoietic progenitors from early murine fetal liver possess hepatic differentiation potential. Am J Pathol. 173, 1818-27 (2008)(PMID:18988804)
  2. Cheung PF et al. Granulin-epithelin precursor is an oncofetal protein defining hepatic cancer stem cells. PLoS One 6, e28246 (2011)(PMID:22194816)

Immunocytochemistry

  1. García-Alonso V et al. Coordinate functional regulation between microsomal prostaglandin E synthase-1 (mPGES-1) and peroxisome proliferator-activated receptor γ (PPARγ) in the conversion of white-to-brown adipocytes. J Biol Chem. 288, 28230-42 (2013)(PMID:23943621)

Immunohistochemistry

  1. Tan KS et al. Localized SCF and IGF-1 secretion enhances erythropoiesis in the spleen of murine embryos. Biol Open 4, 596-607 (2015)(PMID:25887124)
Product category
Research area
Stem cell research
Cancer
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 00:05, Dec 14, 2018 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.