This site is for customers in Asia. Customers in China & other regions, please go to Global page.
HOME > Product search results > Code No. D130-3 Anti-Runx2 (Cbfa1) mAb

Code No. D130-3

Anti-Runx2 (Cbfa1) mAb

Availability (in Japan)

10 or more

(In Japan at 00:05,
Aug 18, 2018 in JST)

Size

100 µg/100 µL

Data
  • Western Blotting

Clonality Monoclonal Clone 8G5
Isotype (Immunized Animal) Mouse IgG2bκ
Applications
WB
1 µg/mL  
IP*
reported.   (PMID: 12231506 / 17786208 / 28507152
IC*
reported.   (PMID: 16818622
IH*
reported.  (PMID: 19124839
ELISA*
reported.  (PMID: 22189971
ChIP*
reported.  (PMID: 17215250 / 21890638
Co-IP*
reported.  (PMID: 25283348
Immunogen (Antigen) Recombinant Runx2
Reactivity [Gene ID]

Human[860], Mouse[12393], Rat*[367218](19124839)

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names runt-related transcription factor 2, CCD, AML3, CCD1, CLCD, OSF2, CBFA1, OSF-2, PEA2aA, PEBP2aA, CBF-alpha-1
Background Runx2, also known as Cbfa1 or PEBP2αA, is an essential transcription factor of skeletal tissues that is involved in the regulation of osteoblast differentiation and bone formation. Runx2-null mice have neither bone tissue nor osteoblasts. FGF receptor signaling, TGF-β, and BMP all activate transcription of Runx2, resulting in inhibition of myogenesis and myogenic differentiation. BMP-induced Runx2 cooperates with BMP-activated Smads to induce osteogenesis. Runx2 has also been shown to play a role in the regulation of chondrocyte hypertrophy and tooth eruption.
Related products D207-3 Anti-Runx mAb
D208-3 Anti-Runx mAb
D234-3 Anti-RUNX3 mAb
D235-3 Anti-RUNX3 mAb
D236-3 Anti-RUNX3 mAb
D127-3 Anti-PEBP2β (CBFβ) (Human) mAb
M077-3 Mouse IgG2b (isotype control)
D134-3 Anti-HES1 mAb
Citations

Western Blotting

  1. Zhang YW et al. A RUNX2/PEBP2alpha A/CBFA1 mutation displaying impaired transactivation and Smad interaction in cleidocranial dysplasia. PNAS. 97, 10549-54 (2000)(PMID:10962029)
  2. Wee HJ et al. Serine phosphorylation of RUNX2 with novel potential functions as negative regulatory mechanisms. EMBO Rep. 3, 967-74 (2002)(PMID:12231506)
  3. Kaneki H et al. Tumor necrosis factor promotes Runx2 degradation through up-regulation of Smurf1 and Smurf2 in osteoblasts. J Biol Chem. 281, 4326-33 (2006)(PMID:16373342)
  4. Lau QC et al. RUNX3 is frequently inactivated by dual mechanisms of protein mislocalization and promoter hypermethylation in breast cancer. Cancer Res. 66, 6512-20 (2006)(PMID:16818622)
  5. Standal T et al. HGF inhibits BMP-induced osteoblastogenesis: possible implications for the bone disease of multiple myeloma. Blood. 109, 3024-30 (2007)(PMID:17138824)
  6. Ohba S et al. Identification of a potent combination of osteogenic genes for bone regeneration using embryonic stem (ES) cell-based sensor. FASEB J. 21, 1777-87 (2007)(PMID:17317722)
  7. Kugimiya F et al. GSK-3beta controls osteogenesis through regulating Runx2 activity. PLoS One. 2, e837 (2007)(PMID:17786208)
  8. Tominaga H et al. CCAAT/enhancer-binding protein beta promotes osteoblast differentiation by enhancing Runx2 activity with ATF4. Mol Biol Cell. 19, 5373-86 (2008)(PMID:18843047)
  9. Underwood KF et al. Regulation of RUNX2 transcription factor-DNA interactions and cell proliferation by vitamin D3 (cholecalciferol) prohormone activity. J Bone Miner Res. 27, 913-25 (2012)(PMID:22189971)
  10. Byun MR et al. Canonical Wnt signalling activates TAZ through PP1A during osteogenic differentiation. Cel Death Differ. 21, 854-63 (2014)(PMID:24510127)
  11. Byun MR et al. (-)-Epicatechin gallate (ECG) stimulates osteoblast differentiation via Runt-related transcription factor 2 (RUNX2) and transcriptional coactivator with PDZ-binding motif (TAZ)-mediated transcriptional activation. J Biol Chem. 289, 9926-35 (2014)(PMID:24515112)
  12. Meyer MB et al. Genomic determinants of gene regulation by 1,25-dihydroxyvitamin D3 during osteoblast-lineage cell differentiation. J Biol Chem. 289, 19539-54 (2014)(PMID:24891508)
  13. Mochin MT et al. Hyperglycemia and redox status regulate RUNX2 DNA-binding and an angiogenic phenotype in endothelial cells. Microvasc Res. 97, 55-64 (2015)(PMID:25283348)
  14. Chen F et al. Nuclear Export of Smads by RanBP3L Regulates Bone Morphogenetic Protein Signaling and Mesenchymal Stem Cell Differentiation. Mol Cell Biol. 35, 1700-11 (2015)(PMID:25755279)
  15. Meyer MB et al. Selective Distal Enhancer Control of the Mmp13 Gene Identified through Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) Genomic Deletions. J Biol Chem. 290, 11093-107 (2015)(PMID:25773540)
  16. Lin ME et al. Runx2 Expression in Smooth Muscle Cells Is Required for Arterial Medial Calcification in Mice. Am J Pathol. 185, 1958-69 (2015)(PMID:25987250)
  17. Ramasnswamy J et al. Inhibition of osteoblast mineralization by phosphorylated phage-derived apatite-specific peptide. Biomaterials. 73, 120-30 (2015)(PMID:26406452)
  18. Jeselsohn R et al. Embryonic transcription factor SOX9 drives breast cancer endocrine resistance. PNAS. 114, E4482-E4491 (2017)(PMID:28507152)
  19. Sun Y et al. Dietary potassium regulates vascular calcification and arterial stiffness. JCI insight 2, e94920 (2017)(PMID:28978809)
  20. Jo YY et al. Bone regeneration is associated with the concentration of tumour necrosis factor-α induced by sericin released from a silk mat. Sci Rep. 7, 15589 (2017)(PMID:29138464)
  21. Xiao Z et al. Polycystin-1 interacts with TAZ to stimulate osteoblastogenesis and inhibit adipogenesis. J Clin Invest. 128, 157-174 (2018)(PMID:29202470)

ChIP

  1. Wang Q et al. Bone morphogenetic protein 2 activates Smad6 gene transcription through bone-specific transcription factor Runx2. J Biol Chem. 282, 10742-8 (2007)(PMID:17215250)
  2. Ohba S et al. Identification of a potent combination of osteogenic genes for bone regeneration using embryonic stem (ES) cell-based sensor. FASEB J. 21, 1777-87 (2007)(PMID:17317722)
  3. Fulzele K et al. Insulin receptor signaling in osteoblasts regulates postnatal bone acquisition and body composition. Cell 142, 309-19 (2010)(PMID:20655471)
  4. Taniguchi N et al. Expression patterns and function of chromatin protein HMGB2 during mesenchymal stem cell differentiation. J Biol Chem. 286, 41489-98 (2011)(PMID:21890638)
  5. Tandon M et al. Runx2 activates PI3K/Akt signaling via mTORC2 regulation in invasive breast cancer cells. Breast Cancer Res. 16, R16 (2014)(PMID:24479521)

Co-IP

  1. Mochin MT et al. Hyperglycemia and redox status regulate RUNX2 DNA-binding and an angiogenic phenotype in endothelial cells. Microvasc Res. 97, 55-64 (2015)(PMID:25283348)

Immunoprecipitation

  1. Wee HJ et al. Serine phosphorylation of RUNX2 with novel potential functions as negative regulatory mechanisms. EMBO Rep. 3, 967-74 (2002)(PMID:12231506)
  2. Ohba S et al. Identification of a potent combination of osteogenic genes for bone regeneration using embryonic stem (ES) cell-based sensor. FASEB J. 21, 1777-87 (2007)(PMID:17317722)
  3. Kugimiya F et al. GSK-3beta controls osteogenesis through regulating Runx2 activity. PLoS One. 2, e837 (2007)(PMID:17786208)
  4. Jeselsohn R et al. Embryonic transcription factor SOX9 drives breast cancer endocrine resistance. PNAS. 114, E4482-E4491 (2017)(PMID:28507152)

Immunocytochemistry

  1. Ali SA et al. A RUNX2-HDAC1 co-repressor complex regulates rRNA gene expression by modulating UBF acetylation. J Cell Sci. 125, 2732-9 (2012)(PMID:22393235)

Immunohistochemistry

  1. Hirata A et al. Localization of runx2, osterix, and osteopontin in tooth root formation in rat molars. J Histochem Cytochem. 57, 397-403 (2009)(PMID:19124839)
  2. Hosoya A et al. Two distinct processes of bone-like tissue formation by dental pulp cells after tooth transplantation. J Histochem Cytochem. 60, 861-73 (2012)(PMID:22899860)
  3. Beier EE et al. Heavy metal lead exposure, osteoporotic-like phenotype in an animal model, and depression of Wnt signaling. Environ Health Perspect. 121, 97-104 (2013)(PMID:23086611)
  4. McDonalod L et al. RUNX2 correlates with subtype-specific breast cancer in a human tissue microarray, and ectopic expression of Runx2 perturbs differentiation in the mouse mammary gland. Dis Model Mech. 7, 525-34 (2014)(PMID:24626992)
  5. Owens TW et al. Runx2 is a novel regulator of mammary epithelial cell fate in development and breast cancer. Cancer Res. 74, 5277-5286 (2014)(PMID:25056120)
  6. Liao L et al. Deletion of Runx2 in Articular Chondrocytes Decelerates the Progression of DMM-Induced Osteoarthritis in Adult Mice. Sci Rep. 7, 2371 (2017)(PMID:28978809)
  7. Xu T et al. Administration of erythropoietin prevents bone loss in osteonecrosis of the femoral head in mice. Mol Med Rep. 16, 8755-8762 (2017)(PMID:29039481)
  8. Ying J et al. Transforming growth factor-beta1 promotes articular cartilage repair through canonical Smad and Hippo pathways in bone mesenchymal stem cells. Life Sci. 192, 84-90(2018)(PMID:29158053)
  9. Kawano T et al. Effects of combined therapy of alendronate and low-intensity pulsed ultrasound on metaphyseal bone repair after osteotomy in the proximal tibia of glucocorticoid-induced osteopenia rats. Osteoporos Sarcopenia.  3, 185-191 (2017)

ELISA

  1. Underwood KF et al. Regulation of RUNX2 transcription factor-DNA interactions and cell proliferation by vitamin D3 (cholecalciferol) prohormone activity. J Bone Miner Res. 27, 913-25 (2012)(PMID:22189971)
Product category
Research area
Cancer
Signal transduction
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 00:05, Aug 18, 2018 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.