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HOME > Product search results > Code No. D050-5 Anti-CD29 (Integrin β1) (Human) mAb-PE

Code No. D050-5

Anti-CD29 (Integrin β1) (Human) mAb-PE

Availability (in Japan)

1-9

(In Japan at 01:30,
Jun 26, 2017 in JST)

Size

1 mL (50 tests)

Data
  • Flow Cytometry

Clonality Monoclonal Clone AG89
Isotype (Immunized Animal) Mouse IgG1
Applications
FCM
20 µL (Ready for use.)  
Immunogen (Antigen) G-361 (Human melanoma cells)
Reactivity [Gene ID]

Human[3688]

Storage buffer PBS/1% BSA/0.09% NaN3
Storage temp. 4°C Conjugate PE Manufacturer MBL
Alternative names ITGB1, integrin beta 1, FNRB, MDF2, VLAB, GPIIA, MSK12, VLA-BETA
Background The integrin family of adhesion molecules participate in important cell-cell and celle-xtracellular matrix interactions in a diverse range of biological processes. Integrins are heterodimers consisting of a a subunit and b subunit. Both a and b subunit are transmembrane proteins with large extracellular domains (>100 kDa for a subunit and >75 kDa for b subunit) that interact with extracellular matrix proteins and relatively small cytoplasmic domains (50 amino acids or less, except for the b4 subunit) that interact with cytoskeletal proteins. The adhesiveness of integrins is dynamically regulated in response to cytoplasmic signals, termed “inside-out” signaling. It has been reported that, upon ligand binding, integrins regulate many intracellular signaling pathways that involve cytoplasmic alkalization, intracellular Ca2+ fluctuation, inositol lipid metabolism, protein kinase C, MAP kinase and phosphatidyl inositol kinase. Anti-integrin monoclonal antibody, AG89, reacts with human integrin b1 chain regardless of the a subunit. AG89 can recognize resting site b1 integrin on the cells, but the reactivity is increased ~2-fold upon integrin activation by anti-activating b1 antibodies and ~3-fold by Mn2+. Furthermore, occupation of the ligand-binding pocket by a soluble ligand (RGD peptide for a5b1 and CS-1 peptide for a4b1) resulted in maximum binding of AG89. The epitope for AG89 lies within residues 426-587.
Related products D050-3 Anti-CD29 (Integrin β1) (Human) mAb
M075-3 Mouse IgG1 (isotype control)
W052-3 Anti-Osteopontin (SPP1) (Human) mAb
D252-3 Anti-CD9 (Human) mAb
K0046-3 Anti-Integrin α7 (Mouse) mAb
Citations

Flow Cytometry

  1. Matsunaga T et al. Potentiated activation of VLA-4 and VLA-5 accelerates proplatelet-like formation. Ann Hematol. 91, 1633-43 (2012)(PMID:22644786)
Product category
Research area
Stem cell research
Cell surface antigens
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 01:30, Jun 26, 2017 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.