Acetylcholinesterase Rapid Staining Kit
◎Nerves and ganglion cells are stained with this kit
◎Easy and Quick procedure
◎Kit utilizes enzymatic reaction of AChE
▼This Kit is used in the following research paper！
Kobayashi, H. et al. ; A new rapid acetylcholinesterase staining kit for diagnosing Hirschsprung's disease. Pediatr Surg Int. 23, 505-508 (2007)
Acetylcholinesterase (AChE) is a tetrameric serine hydrolase that rapidly catalyzes the hydrolysis of
acetylcholine to acetate and choline.
The breakdown of
acetylcholine is critical for the termination of impulse
transmissions at cholinergic synapses within the nervous
system. Progressive loss of cholinergic neurons in
Alzheimer's disease (AD) patients results in severe
memory loss and impairment of cognitive function.
AChE inhibitors are a strategic approach to symptomatic
treatment for AD, since AChE inhibitors increase the levels
of acetylcholine in the synapse, thereby enhancing
cholinergic activity in the affected regions of the brain.
AChE also plays an important role in agriculture since
modifications in AChE can confer resistance to pesticides.
AChE is a key component in many snake venoms, and
AChE staining is routinely used for the initial diagnosis of
Hirschsprung's disease, a congenital disorder caused by the
absence of ganglion cells in the distal colon.
Example of Immunohistochemistry
Myenteric ganglia in normal intestine (x 200)
Submucosal hypertrophic nerve trunks in Hirschsprung's disease (x 400)
The photos are provided by Dr. Kobayashi Hiroyuki, Department of Pediatric Surgery, Juntendo University School of Medicine.
Staining procedure is easy and quick !
＊Please refer to the data sheet for detailed procedure.
||Price (Tax excluded)
||Acetylcholinesterase Rapid Staining Kit
|| 30 tests
- Solution A: 6 mL 1 vial
- Solution B: 6 mL 1 vial
- 3, 3'-diaminobenzidine (DAB) solution: 600 mL 1 vial
- DAB dilution buffer: 12 mL 1 vial
- Disposal droppers: 30 droppers
- Kobayashi, H. et al., Pediatr Surg Int. 23: 505-508, 2007
- Kobayashi H.,et al., Pediatr Surg Int 18:349-353, 2002
- Kobayashi H.,et al., Arch Pathol Lab Med 118:1127-1129, 1994
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