- FRET donor with highly fluorescent quantum yield
- Cell, subcellular structure, or protein labeling
The fluorescent protein Midoriishi-Cyan gene was isolated from the
stony coral Acropora sp.(Midori-ishi in Japanese).
CoralHue® Midoriishi-Cyan 1 (MiCy1) absorbs light maximally at 472 nm
and emits cyan light at 495 nm. It rapidly matures to form a bright dimeric
complex. So it can be used to mark individual cells or to report gene
expression without the problem of protein aggregation. CoralHue®
mMiCy1 is a monomeric version of MiCy1, and can be used to label
proteins or subcellular structures.
These fluorescent proteins are suitable as donor for fluorescence
resonance energy transfer (FRET) because of highly fluorescent
Note: The file is in a tab-delimited text format. It contains values of the wavelength (0.5nm spacing) and brightness (fluorescence intensity peak value normalized to 1). Use a spreadsheet program to create a spectrum that will help you in choosing the appropriate excitation filter, dichroic mirror and fluorescence filter.
|Number of amino acid
|Excit./Emiss. maxima (nm)
|Molar extinction coefficient (M-1cm-1)
||27,250 (472 nm)
||22,150 (470 nm)
|Fluorescence quantum yield
*1Brightness: Molar Extinction Coefficient ×Fluorescence Quantum Yield / 1000
*2Toxicity when expressed in HeLa cells
Monitoring of caspase-3 activity during apoptosis
MiCy and Kusabira-Orange (KO) is a good donor/acceptor pair for FRET (fluorescence resonance energy transfer). FRET based caspase3 monitoring probe is constructed using MiCy and KO, connected by a peptide containing the caspase3 cleavage sequence, DEVD (Asp-Glu-Val-Asp). The activation of caspase3 leads to eliminate FRET by cleavage of DEVD sequence.
Filter, mirror: DM 465HQ, Ex BP440-460HQ, Em BA475-540HQ(cyan), BA555-600HQ(orange)
Lamp: Xe lamp (75W)
Lens: UApo/340 N.A. 1.35
Apoptosis induction: 500 ng/ml of anti-Fas antibody (CH-11; Medical & Biological Laboratories Co.,Ltd.) and 10 μg/ml cycloheximide at 36h post-transfection.
CoralHue® Midori-ishi Cyan can be recognized using antibodies as shown below.
WB: Western blotting, IP: Immunoprecipitation
- Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A, Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer; Biochem J. 381, 307-12(2004) PMID:15065984
※CoralHue® fluorescent proteins were co-developed with the Laboratory
for Cell Function and Dynamics, the Advanced Technology Development
Center, the Brain Science Institute, RIKEN. MBL possesses the license
and deals in the products.
Please send us a completed and signed License Acknowledgement.
Please note that the product will be shipped after we receive a
completed and signed License Acknowledgement (PDF file on the right).
Prior to shipping, our staff may contact you to verify the intended use
of the product. Please also note that a separate agreement or a contract
may be required depending on the intended use.
The product you have ordered is sold for research purpose only. It is
not intended for industrial or clinical use, and shall not be used for
any purposes other than research. You are also asked not to hand over or
resell the product to a third party.
If you belong to a profit organization or a business corporation, or
wish to use the product for profit or commercial purposes, please