- Bright orange fluorescence
- High pH stability
CoralHue® Kusabira-Orange (KO1), from the stony coral Fungia concinna
(Kusabira-ishi in Japanese), absorbs light maximally at 548 nm and emits orange light
at 561 nm. KO1 rapidly matures to form a fluorescent dimeric complex. KO1 can be
used to mark cells or to report gene expression without problems stemming from
CoralHue® monomeric Kusabira Orange (mKO1) maintains the brilliance and pH
stability of the parent protein. mKO1 can be used to label proteins or subcellular
structures or for FRET analysis.
CoralHue® mKO2 is a mutant of mKO1 that features rapid maturation. mKO2 can be used to label proteins or subcellular structures or for reporter assays.
Note: The file is in a tab-delimited text format. It contains values of the wavelength (0.5nm spacing) and brightness (fluorescence intensity peak value normalized to 1). Use a spreadsheet program to create a spectrum that will help you in choosing the appropriate excitation filter, dichroic mirror and fluorescence filter.
|Number of amino acid
|Excit./Emiss. maxima (nm)
|Molar Extinction Coefficient (M-1cm-1)
||73,700 (548 nm)
||51,600 (548 nm)
||63,800 (551 nm)
|Fluorescence quantum yield
|Resistance to PFA fixation
*1Brightness: Molar Extinction Coefficient ×Fluorescence Quantum Yield / 1000
*2Toxicity when expressed in HeLa cells
Kusabira-Orange are useful for labeling organelle.
Kusabira-Orange is easily expressed and detected in a wide range of organisms. It has been demonstrated in nucleoplasm, plasma membrane, endoplasmic reticulum and mitochondrial targeting signal models.
Kusabira-Orange is a useful protein for expression into various species.
KO1 TG pig
Provided by Dr. Nagashima
Cloning and Stem Cells. (2008) 10: 313-324.
mKO1 fused with Vasa protein expressed in Drosophila ovary (red).
Provided by Dr. Nakamura RIKEN
The olfactory bulb of zebrafish
Provided by Dr. Miyasaka and Dr.
Laboratory for Neurobiology of Synapse, BSI, RIKEN
KO1 expressed in the epidermis of onion
Provided by Dr. Iida and Dr. Hoshino
the National Institute of
Basic Biology (NIBB).
CoralHue® KO1, mKO1 and mKO2 can be recognized using antibodies as shown below.
- Anti-monomeric Kusabira-Orange 1 for WB, Code No. M104-3M
- Anti-monomeric Kusabira-Orange 1 for IP, Code No. M105-3M
- Anti-monomeric Kusabira-Orange 2 for WB, IP, IC and IH, Code No. M168-3M
- Anti-monomeric Kusabira-Orange 2 for WB, IP, IC and IH, Code No. PM051M
WB: Western blotting, IP: Immunoprecipitation, IC: Immunocytochemistry, IH: Immunohistochemistry
- Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A, Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer; Biochem J. 381, 307-12(2004) PMID: 15065984
- Matsunari H, Onodera M, Tada N, Mochizuki H, Karasawa S, Haruyama E, Nakayama N, Saito H, Ueno S, Kurome M, Miyawaki A, Nagashima H., Transgenic-Cloned Pigs Systemically Expressing Red Fluorescent Protein, Kusabira-Orange; Cloning and Stem Cells. (2008) 10: 313-324. PMID: 18729767
※CoralHue® fluorescent proteins were co-developed with the Laboratory
for Cell Function and Dynamics, the Advanced Technology Development
Center, the Brain Science Institute, RIKEN. MBL possesses the license
and deals in the products.
Please send us a completed and signed License Acknowledgement.
Please note that the product will be shipped after we receive a
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