Brighter photoconvertible fluorescent protein with high sensitivity
- Photoconvertible fluorescent protein
- Monomer / tetramer
The fluorescent protein Kikume Green gene was isolated from the stony
coral Favia favus (Kikume-ishi in Japanese).
CoralHue® Kikume Green-Red1 (KikGR1) protein emits bright green
fluorescence that can be irreversibly converted to red. KikGR1 can be
photoconverted by irradiation with UV or violet light (350-410 nm).
Photoconverted KikGR1 possesses excitation/emission maxima at
583/593 nm. KikGR1 contains a His62-Tyr63-Gly64 tripeptide sequence
which forms a green chromophore that can be photoconverted to a red
one via formal beta-elimination and subsequent extension of the π-conjugated system.
CoralHue® monomeric Kikume Green-Red (mKikGR1) maintains the
brightness of the parent protein KikGR1. mKikGR1 can be used for
labeling proteins or subcellular structures.
Images are provided by Dr.Tsutsui Hidekazu and Dr. Miyawaki Atsushi,
Laboratory for Cell Function and Dynamics,
Note: The file is in a tab-delimited text format. It contains values of the wavelength (1nm spacing) and brightness (fluorescence intensity peak value normalized to 1). Use a spreadsheet program to create a spectrum that will help you in choosing the appropriate excitation filter, dichroic mirror and fluorescence filter.
|Number of amino acid
|Excit./Emiss. maxima (nm)
|Molar extinction coefficient (M-1cm-1)
||53,700 (507 nm)
||35,100 (583 nm)
||47,100 (504 nm)
||21,750 (579 nm)
|Fluorescence quantum yield
*1Brightness: Molar Extinction Coefficient ×Fluorescence Quantum Yield / 1000
*2Toxicity when expressed in HeLa cells
KikGR1 transgenic zebrafish
The KikGR1 transgenic zebrafish was observed using two-photon excitation microscopy. KikGR1 can be photoconverted using two-photon excitation microscopy with the laser tuned to 780 nm.
Cell tracking using a photoconvertible fluorescent protein.
Hatta K, Tsujii H, Omura T.
Nat Protoc. (2006) 1: 960-967.
CoralHue®Kikume Green-Red can be recognized using antibodies as shown below.
- Anti-Kikume Green-Red for IP, Code No. M129-3M
- Anti-Kikume Green-Red fore WB, Code No. M128-3M
WB: Western blotting, IP: Immunoprecipitation
- Tsutsui H, Karasawa S, Shimizu H, Nukina N, Miyawaki A. Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. (2005) 6: 233-238 . PMID: 15731765
- Hatta K, Tsujii H, Omura T. Cell tracking using a photoconvertible fluorescent protein. Nat Protoc. (2006) : 960-967. PMID: 17406330
- Habuchi S, Tsutsui H, Kochaniak AB, Miyawaki A, van Oijen AM. van Oijen mKikGR, a Monomeric Photoswitchable Fluorescent Protein. PLoS ONE (2008) 3: e3944 PMID: 19079591
- Tsutsui H, Shimizu H, Mizuno H, Nukina N, Furuta T, Miyawaki A. The E1 mechanism in photo-induced beta-elimination reactions for green-to-red conversion of fluorescent proteins. Chem Biol. (2009) 16:1140-7. PMID: 19942137
- Mizuno H, Dedecker P, Ando R, Fukano T, Hofkens J, Miyawaki A Higher resolution in localization microscopy by slower switching of a photochromic protein. Photochem Photobiol Sci. (2010) 2: 239-248. PMID: 20126801
※CoralHue® Kikume Green-Red is the product licensed to MBL affiliates, for patent application JP 2005-507027 and/or foreign equivalents owned by the Institute of Physical and Chemical Research (RIKEN)
※CoralHue® fluorescent proteins were co-developed with the Laboratory
for Cell Function and Dynamics, the Advanced Technology Development
Center, the Brain Science Institute, RIKEN. MBL possesses the license
and deals in the products.
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