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Azami-Green


  • Bright green fluorescence
  • High pH stability
  • Monomer/tetramer

CoralHue® Azami-Green (AG), cloned from the stony coral Galaxea fascicularis (Azami-sango in Japanese), absorbs light maximally at 492 nm and emits green light at 505 nm. AG is stable in the biological pH range and does not show a significant loss of fluorescent signal, making it advantageous over other fluorescent proteins such as EGFP. And AG also matures rapidly to form tetramers that are brighter than EGFP.
CoralHue® monomeric Azami-Green (mAG1) maintains the brightness and pH stability of the parent protein AG. mAG1 can be used for labeling proteins or subcellular structures.


Fluorescent properties

AG

mAG

fluorescent properties

Azami Green(AG) spectrum data files (text files)
AG excitation (8K)  AG emission (8K)
mAG1 excitation (8K)  mAG1 emission (8K)

Note: The file is in a tab-delimited text format. It contains values of the wavelength (0.5nm spacing) and brightness (fluorescence intensity peak value normalized to 1). Use a spreadsheet program to create a spectrum that will help you in choosing the appropriate excitation filter, dichroic mirror and fluorescence filter.

CHARACTERISTIC AG mAG1
Oligomerization Tetramer Monomer
Number of amino acid 225 225
Excit./Emiss. Maxima (nm) 492/505 492/505
Molar extinction coefficient (M-1cm-1) 72,300 (492 nm) 55,500 (492 nm)
Fluorescence quantum yield 0.67 0.74
Brightness *1 48.4 41.0
pH sensitivity pKa<5.0 pKa=5.8
Cytotoxicity *2 No No

*1Brightness: Molar Extinction Coefficient ×Fluorescence Quantum Yield / 1000
*2Toxicity when expressed in HeLa cells

 

Performance and use

AG is resistant to acidity

AG resistant to acidityAG shows high resistance in a wide range of biological pH. This allows AG to be used identify cells or to report gene expression without being influenced by pH change. The pH dependence of both CoralHue® AG and mAG1 was identical to that of its absorption spectra.



AG and mAG1 are useful for labeling organelle.

AG and mAG1 performance in fusions has been demonstrated in the nucleoplasm, plasma membrane, ER and mitochondria-targeting signal models. We offer these constructs as targeting vector series.

mAG1 is available for protein labeling

mAG1 performance in fusion has been demonstrated in p80 coilin, SC35 and fibrillarin.

AG resistant to acidity

Recommended antibodies

CoralHue® AG and mAG1 can be recognized using antibodies as shown below.

  • Anti-monomeric Azami-Green 1 for WB, Code No. M102-3M
  • Anti-Azami-Green for WB, Code No. PM011M
  • Anti-Azami-Green for IP, Code No. M103-3M
  • Anti-monomeric Azami-Green 1 for WB, IP, IC and IH. Code No. PM052M

WB: Western blotting, IP: Immunoprecipitation, IC: Immunocytochemistry, IH: Immunohictochemistry

References

  • Karasawa S, Araki T, Yamamoto-Hino M, Miyawaki A, A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling; J Biol Chem. 278, 34167-34171 (2003) PMID: 12819206
  • Ebisawa T, Yamamura A, Kameda Y, Hayakawa K, Nagata K, Tanokura M. Crystallization and preliminary X-ray analysis of a monomeric mutant of Azami-Green (mAG), an Aequorea victoria green fluorescent protein-like green-emitting fluorescent protein from the stony coral Galaxea fascicularis. Acta Crystallogr Sect F Struct Biol Cryst Commun. 65(Pt 12):1292-5. (2009) PMID: 20054132

CoralHue® fluorescent proteins were co-developed with the Laboratory for Cell Function and Dynamics, the Advanced Technology Development Center, the Brain Science Institute, RIKEN. MBL possesses the license and deals in the products.


Please send us a completed and signed License Acknowledgement.

Please note that the product will be shipped after we receive a completed and signed License Acknowledgement (PDF file on the right). Prior to shipping, our staff may contact you to verify the intended use of the product. Please also note that a separate agreement or a contract may be required depending on the intended use.
The product you have ordered is sold for research purpose only. It is not intended for industrial or clinical use, and shall not be used for any purposes other than research. You are also asked not to hand over or resell the product to a third party.
If you belong to a profit organization or a business corporation, or wish to use the product for profit or commercial purposes, please contact us.


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