CoralHue™ Azami-Green (AG), cloned from the stony coral Galaxea fascicularis (Azami-sango in Japanese), absorbs light maximally at 492 nm and emits green light at 505 nm. AG is stable in the biological pH range and does not show a significant loss of fluorescent signal, making it advantageous over other fluorescent proteins such as EGFP. And AG also matures rapidly to form tetramers that are brighter than EGFP.
CoralHue™ monomeric Azami-Green (mAG1) maintains the brightness and pH stability of the parent protein AG. mAG1 can be used for labeling proteins or subcellular structures.
|Azami Green(AG) spectrum data files (text files)|
|AG excitation (8K)||AG emission (8K)|
|mAG1 excitation (8K)||mAG1 emission (8K)|
Note: The file is in a tab-delimited text format. It contains values of the wavelength (0.5nm spacing) and brightness (fluorescence intensity peak value normalized to 1). Use a spreadsheet program to create a spectrum that will help you in choosing the appropriate excitation filter, dichroic mirror and fluorescence filter.
|Number of amino acid||225||225|
|Excit./Emiss. Maxima (nm)||492/505||492/505|
|Molar extinction coefficient (M-1cm-1)||72,300 (492 nm)||55,500 (492 nm)|
|Fluorescence quantum yield||0.67||0.74|
*1Brightness: Molar Extinction Coefficient ×Fluorescence Quantum Yield / 1000
*2Toxicity when expressed in HeLa cells
AG shows high resistance in a wide range of biological pH. This allows AG to be used identify cells or to report gene expression without being influenced by pH change. The pH dependence of both CoralHue™ AG and mAG1 was identical to that of its absorption spectra.
AG and mAG1 performance in fusions has been demonstrated in the nucleoplasm, plasma membrane, ER and mitochondria-targeting signal models. We offer these constructs as targeting vector series.
mAG1 performance in fusion has been demonstrated in p80 coilin, SC35 and fibrillarin.
CoralHue™ AG and mAG1 can be recognized using antibodies as shown below.
WB: Western blotting, IP: Immunoprecipitation, IC: Immunocytochemistry, IH: Immunohictochemistry
※CoralHue™ fluorescent proteins were co-developed with the Laboratory for Cell Function and Dynamics, the Advanced Technology Development Center, the Brain Science Institute, RIKEN. MBL possesses the license and deals in the products.
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