Fluorometric Phosphatase Assay Kits

CycLex Protein Phosphatase Cdc25 Fluorometric Assay Kit is a fluorometric and non-radioactive assay designed to measure the activity of Cdc25A,B,C protein phosphatase.
Activation of cyclin-dependent kinases in higher eukaryotic cells can be achieved through dephosphorylation of two conserved residues, Thr14 and Tyr15 by members of the Cdc25 phosphatase family, Cdc25A, Cdc25B and Cdc25C. The Cdc25 dual-specificity phosphatases control progression through the eukaryotic cell division cycle by activating cyclin-dependent kinases. Cdc25A plays an important role at the G1/S-phase transition. Cdc25A degradation during mitotic exit and in early G1 is mediated by the anaphase-promoting complex or cyclosome (APC/C)(Cdh1) ligase, and that a KEN-box motif in the N-terminus of the protein is required for its targeted degradation. Cdc25B undergoes activation during S-phase and plays a role in activating the mitotic kinase Cdk1/cyclin B in the cytoplasm. Active Cdk1/cyclin B then phosphorylates and activates Cdc25C leading to a positive feedback mechanism and to entry into mitosis. In addition to their essential function in the normal cell cycle, cdc25 phosphatases are involved in the checkpoint-induced control of cell cycle progression. Finally, Cdc25A and B phosphatases have been shown to possess an important oncogenic potential and to be overexpressed in a variety of cancers and cancer cell lines.

Protein Phosphatase Cdc25 Combo Fluorometric Assay Kit　

CycLex Protein Phosphatase LMW-PTP / ACP1 Fluorometric Assay Kit is a fluorometric and non-radioactive assay designed to measure the activity of LMW-PTP/ACP1 protein phosphatase.
The low molecular weight protein tyrosine phosphatase (LMW-PTP) is an 18-kDa cytosolic enzyme, also known as acidic protein phosphatase 1 (ACP1). LMW-PTP/ACP1 is specific for phosphotyrosine in peptides and proteins, but the enzyme shares very limited sequence homology with other PTPases. Recent studies suggested that entopic overexpression of LMW-PTP/ACP1 is sufficient to confer transformation in epithelial cells and its oncogenic activities required EphA2. LMW-PTP/ACP1 negatively regulates EphA2 receptor tyrosine kinase. LMW-PTP/ACP1 is a positive regulator of both tumor onset and development through ephrin- EphA2 signaling process, and it is a potential target of anticancer drug development.

Protein Phosphatase LMW-PTP / ACP1 Fluorometric Assay Kit