Mice used in each experiment are being reared under controlled light-dark conditions. When the light-dark cycle is 12/12 hours, the starting time of the light period is “ZT0” and the starting time of the dark period is “ZT12”. “ZT” is the abbreviation for “Zeitgeber Time”. “Zeitgeber” in German is “Time giver” in English and refers to the “agent of entrainment”.
The Dbp gene is regulated by the BMAL1/CLOCK complex and expressed rhythmically. DBP binds to a D-box motif in the promoter region of CCGs, and positively regulates its expression, while E4BP4 negatively regulates CCG expression via binding to D-Box of CCGs. DBP is expressed antiphase to E4BP4 . It is also known that DBP and E4BP4 bind to a D-box in the promoter of Per1, an upstream clock factor, regulating Per1 expression.
This is a monoclonal antibody against NFIL3 (E4BP4), an essential transcription factor for the development of innate lymphoid cells.
* Note Because the Fc region of the antibody was genetically changed to one of rabbit’s immunoglobulin, please use anti-rabbit IgG secondary antibody.
Mouse bone marrow-derived mast cells (left) and the mouse helper T cell line D10.G4.1 (right) were stained with this product (Code No. M225-3).
Using nuclear extract derived from mouse liver (ZT2), WB was performed.
Using the mouse helper T cell line D10.G4.1, immunoprecipitation was performed.
E4BP4 (NFIL3) and immunity
NFIL3/E4BP4, a leucine zipper type transcription factor, is known to play various roles in the immune system. It inhibits excessive T cell activation through the production of IL-10, an anti-inflammatory cytokine, and is involved in IgE class switching in B cells. Recently it was found to be essential for the development of innate lymphoid cells involved in natural immunity.