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HOME > Product search results > Code No. CY-7055 CycLex® Total p62 ELISA Kit

Code No.　CY-7055

CycLex® Total p62 ELISA Kit

Price

¥107,800

Availability (in Japan)

1-9

（In Japan at 00:05,
Apr 20, 2024 in JST）

Size

96 Assay

Data

  • Example of Test Results (HeLa cells)

    Example of Test Results (HeLa cells)
    Measurement of total p62 (by Cat#CY-7055), phospho-p62 Ser349 (by Cat#CY-7056), and Ser403 (by Cat#CY-7057) in cell lysates of HeLa cells treated with 0-25 nM Bafilomycin A1 and/or 12.5-100 nM Rapamycin for 17 hours.

  • Example of Test Results (MEF cells)

    Example of Test Results (MEF cells)
    Measurement of total p62 (by Cat#CY-7055), phospho-p62 Ser349 (by Cat#CY-7056), and Ser403 (by Cat#CY-7057) in cell lysates of MEF (mouse embryonic fibroblast) treated with 0-25 nM Bafilomycin A1 and/or 50-100 nM Rapamycin and/or for 17 hours.
Components Microplate, 10X Wash Buffer, 50X Phosphatase Inhibitor, 1st Lysis Buffer, 2nd Lysis Buffer, p62 standard, Dilution Buffer, HRP conjugated Detection Antibody, Substrate Reagent, Stop Solution
Intended use This kit is used for the quantitative measurement of human or mouse p62 protein in cell lysate.
Storage temp. 4°C Manufacturer MBL
Sample Cell lysate
Measurement range 1.5 - 96 ng/mL
Sensitivity 0.154 ng/mL～
Alternative names sequestosome 1, p60, p62, A170, OSIL, PDB3, ZIP3, p62B/Osi, A170, STAP, OSF-6/Osi, ZIP
Background The sequstosome1, p62/SQSTM1, is composed of multiple domain, which is Phox/Bem domain1, ZZ-type zinc finger domain, TRAF6 binding domain, LC3-intoracting region (LIR) motif, Keap1-interacting region (KIR), and ubiquitin-associated domain (UBA). Thus p62
Related products PM045 Anti-p62 (SQSTM1) pAb
PM074 Anti-Phospho-p62 (SQSTM1) (Ser351) pAb
M162-3 Anti-p62 (SQSTM1) (Human) mAb
M217-3 Anti-Phospho-p62 (SQSTM1) (Ser351) mAb
D343-3 Anti-Phospho-p62 (SQSTM1) (Ser403) mAb
D344-3 Anti-Phospho-p62 (SQSTM1) (Ser403) mAb
PM066 Anti-p62 C-terminal pAb
M162-A48 Anti-p62 (SQSTM1) (Human) mAb-Alexa Fluor™ 488
M162-A59 Anti-p62 (SQSTM1) (Human) mAb-Alexa Fluor™ 594
M162-A64 Anti-p62 (SQSTM1) (Human) mAb-Alexa Fluor™ 647
Citations
  1. Ikeda S et al. Hypoxia-inducible hexokinase-2 enhances anti-apoptotic function via activating autophagy in multiple myeloma. Cancer Sci. 111, 4088-4101 (2020) (PMID:32790954)
Product category
Research area
Autophagy
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