Antibody detailed product information

Code No.	RN013MW
Anti-Nono (P54NRB) mAb
Price	¥52,800
Size	100 µL (1 mg/mL)
Availability (in Japan)	1-9 （In Japan at 17:00, Apr 18, 2024 in JST）
Clonality	Monoclonal
Clone	C5
Isotype (Immunized Animal)	Mouse IgG2aκ
Applications	WB　1 µg/mL IP　2-5 µg/sample IC　10 μg/mL IH　5 μg/mL
Immunogen (Antigen)	KLH-conjugated synthetic peptide, CPPAFNRPAPGAE (corresponding to amino acid residues 453-464 of mouse Nono)
Reactivity [Gene ID]	Human(-), Mouse[53610], Rat[317259], Hamster[100757453]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Alternative names	non-POU domain-containing octamer-binding protein nonA, NRB54, AA407051, AV149256
Background	Nono, also known as p54nrb, localizes to nuclear paraspeckles composed of distinct membrane-less subnuclear organelles termed as nuclear bodies. Paraspeckles consist of some RNAs and proteins, and representative RNA component of paraspeckle is Neat1 long non-coding RNA, which can bind to Nono directly. Nono plays multiple roles in gene expression regulation systems such as transcription and splicing by binding to DNA and RNA via its coiled coil domain and RNA recognition motif, respectively. It is also reported that Nono relates to nuclear retention of “A-to-I edited” RNA.
Related products	RN014MW Anti-SFPQ (PSF) mAb RN092PW Anti-NONO (P54NRB) pAb M019-3 Anti-Nucleolin mAb M041-3 Anti-PML (Human) mAb PM064 Anti-Lamin B1 pAb PM091 Anti-Per1 (Mouse) pAb PM093 Anti-NR1D2 (Rev-erbβ) pAb
Product category	Research area:RNA-RNP Network Epigenetics Brands:RiboCluster Profiler™
Data
References	Imamura K et al. Long noncoding RNA NEAT1-dependent SFPQ relocation from promoter region to paraspeckle mediates IL8 expression upon immune stimuli. Mol Cell 53, 393-406 (2014) (PMID:24507715) Hirose T et al. NEAT1 long noncoding RNA regulates transcription via protein sequestration within subnuclear bodies. Mol Biol Cell 25,169-83 (2014) (PMID:24173718) Elzbieta K et al. Distinct roles of DBHS family members in the circadian transcriptional feedback loop. Mol Cell Biol. 32, 4585-94 (2012) (PMID:22966205) Nakagawa S, Hirose T. Paraspeckle nuclear bodies--useful uselessness? Cell Mol Life Sci. 69, 3027–36 (2012) (PMID:22476590) Nakagawa S et al. Paraspeckles are subpopulation-specific nuclear bodies that are not essential in mice. J Cell Biol. 193, 31-9 (2011) (PMID:21444682) Kuwahara S et al. PSPC1, NONO, and SFPQ are expressed in mouse Sertoli cells and may function as coregulators of androgen receptor-mediated transcription. Biol Reprod. 75, 352-9 (2006) (PMID:16641145) Fox AH et al. Paraspeckles: a novel nuclear domain. Curr Biol. 12, 13-25 (2002) (PMID:11790299)

The availability is based on the information in Japan at 17:00, Apr 18, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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