Antibody detailed product information
| Code No. | PM077 | |
| Anti-5-hydroxymethylcytosine (5hmC) pAb | ||
| Anti-5-hydroxymethylcytosine (5hmC) pAb is useful for dot blot and hMeDIP. 5hmC is enriched in brain, liver and stem cells and may function as a regulator of gene activation. | ||
| Price | ¥66,000 | |
| Size | 100 µL | |
| Availability (in Japan) | 10 or more
（In Japan at 00:05, Apr 20, 2024 in JST） |
|
| Clonality | Polyclonal | |
| Clone | Polyclonal | |
| Isotype (Immunized Animal) |
Rabbit Ig (aff.) | |
| Applications | IP 2 μL/sample IH Can be used. (paraffin section) DB 1:5,000 |
|
| Immunogen (Antigen) |
BSA-conjugated 5-hydroxymethylcytidine | |
| Storage buffer | PBS/50% glycerol, pH 7.2 | |
| Storage temp. | -20°C | |
| Conjugate | Unlabeled | |
| Manufacturer | MBL | |
| Alternative names | 5-hmC | |
| Background | DNA methylation is one of many critical epigenetic modifications. In most cases, cytosine (C) in DNA sequence is converted to 5-methylcytosine (5mC) with addition of a methyl group by the enzymatic activity of DNA methyltransferase. DNA methylation plays a role in the regulation of gene expression. It has been reported that abnormal DNA methylation level is involved in many diseases.
5-hydroxymethylcytosine (5hmC), a newly identified epigenetic modification following 5mC, is generated from oxidation of 5mC by the enzymatic activity of TET family proteins. 5hmC may function as a regulator of gene expression as well as 5mC. |
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| Related products | MI-11-3 Anti-Bromodeoxyuridine mAb MI-11-5 Anti-Bromodeoxyuridine mAb-PE D345-3 Anti-1-methyladenosine (m1A) mAb D346-3 Anti-5-methylcytidine (m5C) mAb PM035 Normal Rabbit IgG PM085 Anti-ZBTB24 pAb PM086 Anti-ZBTB24 (Human) pAb D347-3 Anti-Pseudouridine mAb M227-3 Anti-Digoxigenin (DIG) mAb M218-3 Anti-5-hydroxymethylcytosine (5hmC) mAb M228-3 Anti-FITC mAb RN019M Anti-2,2,7-trimethylguanosine (m3G/TMG) mAb RN016M Anti-7-methylguanosine (m7G)-Cap mAb RN017M Anti-7-methylguanosine (m7G) mAb PM098 Anti-Inosine pAb |
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| Product category | Research area:Epigenetics | |
| Data |    |
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- The availability is based on the information in Japan at 00:05, Apr 20, 2024 in JST.
- The special price is shown in red color.
- Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
- Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization - For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
- For storage temparature: RT: room temparature
- Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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