Antibody detailed product information

Code No. PM044
Anti-PIWIL2 (MILI) (Mouse) pAb
Price ¥52,800
Size 100 µL
Availability (in Japan) 10 or more
（In Japan at 00:05, Apr 24, 2024 in JST）
Clonality Polyclonal
Clone Polyclonal
Isotype
(Immunized Animal) 		Rabbit Ig (aff.)
Applications WB　1:1,000  
IP　1 µL/sample  
IH*　reported.  （PMID: 29018194）
RIP*　reported.  （PMID: 18381894 / 19735482 / 20439430）
Immunogen
(Antigen)		 synthetic peptide VRKDREEPRSSLPDPS corresponding to Mouse PIWIL2 (107-122 a.a.)
Reactivity [Gene ID]

Mouse[57746]
Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Background The Piwi/Argonaute protein family plays important roles in stem-cell self-renewal, RNA silencing and translational regulation in various organisms. The family members are defined by conserved both PAZ (Piwi Argonaut and Zwille) and Piwi domains participate in RNA binding. Piwi-interacting RNA (piRNA) is a class of small RNA molecules that is expressed uniquely in mammalian testes and forms RNA-protein complexes with Piwi proteins. These small regulatory RNAs have been implicated in spermatogenesis, repression of retrotransposon transposition in germline cells, epigenetic regulation and positive regulation of translation and mRNA stability. MIWI (PIWIL1) and MILI (PIWIL2) are mouse homologues of Piwi. MILI is expressed from primordial germ cells (PGC) in embryo, whereas MIWI is detected later from the mid-pachytene stage to early round spermatids. Their expression overlaps in the mid-pachytene and early round spermatid stage. The expression patterns and knockout phenotypes of mammalian Piwi members indicated MILI is an essential factor for meiotic differentiation during spermatogenesis.
Related products RN003M Anti-EIF2C2 (AGO2) (Human) mAb
RN1005 RIP-Assay Kit for microRNA
D128-3 Anti-Phospho-STAT3 (Tyr708) mAb
M207-3 Anti-MitoPLD (Pld6) mAb
D289-3 Anti-Np95 (Uhrf1) (Mouse) mAb
D356-3 Anti-Jmjd1c (Mouse) mAb
M202-3 Anti-Np95 (Uhrf1) mAb
RN125PW Anti-HENMT1 pAb
D357-3 Anti-Ace (CD143) (Mouse) mAb
D363-3 Anti-Ly6k (Mouse) mAb
D365-3 Anti-PIWIL2 (MILI) (Mouse) mAb
Product category Research area:Stem cell research RNA-RNP Network Epigenetics Cancer
Data

Citations

Western Blotting

  1. Kojima K et al. Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells. Genes Cells. 14, 1155-65 (2009)(PMID:19735482)
  2. Kuramochi-Miyagawa S et al. MVH in piRNA processing and gene silencing of retrotransposons. Genes Dev. 24, 887-92 (2010)(PMID:20439430)
  3. Ding D et al. PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice. Nat Commun. 8, 819 (2017)(PMID:29018194)
  4. Ding D et al. TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice. Nat Commun. 9, 127 (2017)(PMID:29317670)
  5. Ding D et al. Mitochondrial membrane-based initial separation of MIWI and MILI functions during pachytene piRNA biogenesis. Nucleic Acids Res. 47, 2594-2608 (2019) (PMID:30590800)

Immunoprecipitation

  1. Kojima K et al. Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells. Genes Cells. 14, 1155-65 (2009)(PMID:19735482)
  2. Shiromoto Y et al. GPAT2, a mitochondrial outer membrane protein, in piRNA biogenesis in germline stem cells. RNA 19, 803-10 (2013)(PMID:23611983)

Immunohistochemistry

  1. Ding D et al. PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice. Nat Commun. 8, 819 (2017)(PMID:29018194)
  2. Ding D et al. TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice. Nat Commun. 9, 127 (2017)(PMID:29317670)
  3. Ding D et al. Mitochondrial membrane-based initial separation of MIWI and MILI functions during pachytene piRNA biogenesis. Nucleic Acids Res. 47, 2594-2608 (2019)(PMID:30590800)

RNP Immunoprecipitation

  1. Kuramochi-Miyagawa S et al. DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes. Genes Dev. 22, 908-17 (2008)(PMID:18381894)
  2. Kojima K et al. Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells. Genes Cells. 14, 1155-65 (2009)(PMID:19735482)
  3. Kuramochi-Miyagawa S et al. MVH in piRNA processing and gene silencing of retrotransposons. Genes Dev. 24, 887-92 (2010)(PMID:20439430)
  4. Ding D et al. PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice. Nat Commun. 8, 819 (2017)(PMID:29018194)
  5. Ding D et al. TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice. Nat Commun. 9, 127 (2017)(PMID:29317670)
  6. Nishimura T et al. PNLDC1, mouse pre-piRNA Trimmer, is required for meiotic and post-meiotic male germ cell development. EMBO Rep. 19, e44957 (2018)(PMID:29444933)
  7. Ding D et al. Mitochondrial membrane-based initial separation of MIWI and MILI functions during pachytene piRNA biogenesis. Nucleic Acids Res. 47, 2594-2608 (2019)(PMID:30590800)
  • The availability is based on the information in Japan at 00:05, Apr 24, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
MBLCopyright (C)2015 Medical ＆ Biological Laboratories Co., Ltd. All Rights Reserve