Code No. |
PM032 |
Print out |
Anti-His-tag pAb
|
Price |
¥40,000
|
Size |
100 µL |
Availability (in Japan) |
10 or more
(In Japan at 00:05, Mar 29, 2024 in JST)
|
Clonality |
Polyclonal |
Clone |
Polyclonal |
Isotype
(Immunized Animal) |
Rabbit Ig (aff.) |
Applications |
WB 1:1,000 IP 5 µL/sample IC* reported. (PMID: 21216843)
|
Immunogen
(Antigen) |
Carrier protein conjugated 6xHissynthetic peptide |
Storage buffer |
PBS/50% glycerol, pH 7.2 |
Storage temp. |
-20°C |
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Background |
Expression vectors containing a protein and a tag peptide are commonly used. His-tag fusion protein expression system is preferably used in various laboratories, because its simple protein purification step by heavy metal, such as nickel, affinity chromatography. This specific antibody for His-tag fusion protein is useful tool for monitoring of the fusion protein expression and affinity purification. |
Related products |
PM032-8 Anti-His-tag pAb-Agarose D291-3 Anti-His-tag mAb D291-6 Anti-His-tag mAb-Biotin D291-7 Anti-His-tag mAb-HRP-DirecT D291-8 Anti-His-tag mAb-Agarose D291-10 Anti-His-tag mAb-Magnetic Agarose D291-A48 Anti-His-tag mAb-Alexa Fluor™ 488 D291-A59 Anti-His-tag mAb-Alexa Fluor™ 594 D291-A64 Anti-His-tag mAb-Alexa Fluor™ 647 M089-3 Anti-His-tag mAb M136-3 Anti-His-tag mAb D291-11 Anti-His-tag mAb-Magnetic Beads
|
Product category |
Tools:Epitope tags |
Data |
|
Citations |
Western Blotting - Shibata Y et al. Activation of the IκEB kinase complex by HTLV-1 Tax requires cytosolic factors involved in Tax-induced polyubiquitination. J Biochem. 150, 679-86 (2011)(PMID:21862596)
- Kajikawa M et al. The intertransmembrane region of Kaposi's sarcoma-associated herpesvirus modulator of immune recognition 2 contributes to B7-2 downregulation. J Virol. 86, 5288-96 (2012)(PMID:22379101)
- Nishiyama A et al. Uhrf1-dependent H3K23 ubiquitylation couples maintenance DNA methylation and replication. Nature. 502, 249-53 (2013)(PMID:24013172)
- Lee Y et al. Structure of the triose-phosphate/phosphate translocator reveals the basis of substrate specificity. Nat Plants. 3, 825-832 (2017)(PMID:28970497)
- Li Z et al. Destabilization of linker histone H1.2 is essential for ATM activation and DNA damage repair. Cell Res. 28, 756-770 (2018)(PMID:29844578)
- Fukui R et al. Cleavage of Toll-Like Receptor 9 Ectodomain Is Required for In Vivo Responses to Single Strand DNA. Front Immunol. 9, 1491 (2018)(PMID:29997629)
- Kang J et al. FIH permits NAA10 to catalyze the oxygen-dependent lysyl-acetylation of HIF-1α. Redox Biol. 19, 364-374 (2018)(PMID:30237125)
- Li M et al. p53 cooperates with SIRT6 to regulate cardiolipin de novo biosynthesis. Cell Death Dis. 9, 941 (2018)(PMID:30237540)
- Gao T et al. PKCζ Phosphorylates SIRT6 to Mediate Fatty Acid β-Oxidation in Colon Cancer Cells. Neoplasia. 21, 61-73 (2019)(PMID:30504065)
- Kim J et al. Ketohexokinase-A acts as a nuclear protein kinase that mediates fructose-induced metastasis in breast cancer. Nat Commun. 11, 5436 (2020)(PMID:33116123)
Immunoprecipitation - Kojima K et al. Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells. Genes Cells. 14, 1155-65 (2009)(PMID:19735482)
- Zhang L et al. POH1 deubiquitinates pro-interleukin-1β and restricts inflammasome activity. Nat Commun. 9, 4225 (2018)(PMID:30315153)
Immunocytochemistry - Takemoto M et al. Laminar and areal expression of unc5d and its role in cortical cell survival. Cereb Cortex. 21, 1925-34 (2011)(PMID:21216843)
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