Antibody detailed product information

Code No.	PM003-8
Anti-V5-tag pAb-Agarose
Price	¥52,800
Size	Gel:200 µL (Slurry:400 µL)
Availability (in Japan)	10 or more （In Japan at 00:05, Apr 20, 2024 in JST）
Clonality	Polyclonal
Clone	Polyclonal
Isotype (Immunized Animal)	Rabbit Ig (aff.)
Applications	IP　20 µL of gel slurry
Immunogen (Antigen)	Carrier protein conjugated synthetic peptide (CP-GKPIPNPLLGLDST)
Storage buffer	400 µg of anti-V5-tag polyclonal antibody covalently coupled to 200 µL of agarose gel and provided as a 50% gel slurry (in PBS/0.09% NaN₃)
Storage temp.	4°C
Conjugate	Agarose
Manufacturer	MBL
Background	Expression vectors containing a protein and a tag peptide are commonly used. V5-tag fusion protein expression system is preferably used in various laboratories. This specific antibody for V5-tag fusion protein is a useful tool for monitoring of the fusion protein expression and affinity purification.
Related products	PM003 Anti-V5-tag pAb M167-3 Anti-V5-tag mAb M167-10 Anti-V5-tag mAb-Magnetic Agarose 3315-205 V5-tag peptide M215-7 Anti-V5-tag mAb-HRP-DirecT M215-6 Anti-V5-tag mAb-Biotin 3318 V5-tagged Protein Purification Gel Ver.2 3317A V5-tagged Protein Purification Kit Ver.2 (Trial Kit) 3317 V5-tagged Protein Purification Kit Ver.2 3341 V5-tagged Protein Magnetic Purification Kit M167-11 Anti-V5-tag mAb-Magnetic Beads M215-3 Anti-V5-tag mAb M217-3 Anti-Phospho-p62 (SQSTM1) (Ser351) mAb M215-11 Anti-V5-tag mAb-Magnetic Beads
Product category	Tools:Epitope tags
Data
Citations	Co-IP Uenaka T et al. In silico drug screening by using genome-wide association study data repurposed dabrafenib, an anti-melanoma drug, for Parkinson's disease. Hum Mol Genet. 27, 3974-3985 (2018)(PMID:30137437) Wu X et al. JOSD1 inhibits mitochondrial apoptotic signalling to drive acquired chemoresistance in gynaecological cancer by stabilizing MCL1. Cell Death Differ. In press(PMID:31043700) Taira J et al. Dephosphorylation of clustered phosphoserine residues in human Grb14 by protein phosphatase 1 and its effect on insulin receptor complex formation. J Pept Sci. 25, e3207 (2019)(PMID:31347216)

The availability is based on the information in Japan at 00:05, Apr 20, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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