Antibody detailed product information

Code No. M216-3
Anti-TERT (Human) mAb
Price ¥74,800
Size 100 µL (1 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 17:00, Apr 19, 2024 in JST）
Clonality Monoclonal
Clone 10E9-2
Isotype
(Immunized Animal) 		Mouse IgG2bκ
Applications WB　0.5-10 µg/mL  （PMID: 24550003）
IP*　reported.  （PMID: 24550003 / 26830230）
IC*　reported.  （PMID: 24550003）
ChIP*　Can be used.  （PMID: 24550003）
Immunogen
(Antigen)		 Recombinant C-terminal His-tagged hTERT protein of 157 amino acids of hTERT (position 304 to 460)
Reactivity [Gene ID]

Human[7015](PMID: 24550003)
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names TP2, TRT, CMM9, EST2, TCS1, hTRT, DKCA2, DKCB4, PFBMFT1
Background Human telomerase reverse transcriptase (hTERT) is 1132 amino acids, 127 kDa protein, that is the catalytic subunit of the telomerase. Telomerase is an RNA-dependent DNA polymerase that catalyzes the addition of telomeric repeat unit, 5'-TTAGGG-3', to chromosome ends. Human telomerase consists of three major subunits: TERT, a template RNA called hTR, and telomerase-associated protein (TEP-1). hTERT and hTR are minimally required to reconstitute telomerase activity in vitro. In these subunits, hTR is constitutively expressed; TERT transcription is a primary mechanism for regulation of telomerase activity. In most human somatic cells, telomerase activity is undetectable, whereas approximately 85% of human cancers, including breast, prostate, stomach, bladder, colon, and liver cancer, have telomerase activity. Activation of telomerase is implicated in cell immortalization and cancer cell pathogenesis.
Related products M077-3 Mouse IgG2b (isotype control)
Product category Research area:Epigenetics Cancer
Data


Citations

Western Blotting

  1. Maida Y et al. Involvement of telomerase reverse transcriptase in heterochromatin maintenance. Mol Cell Biol. 34, 1576-93 (2014)(PMID:24550003)

ChIP

  1. Maida Y et al. Involvement of telomerase reverse transcriptase in heterochromatin maintenance. Mol Cell Biol. 34, 1576-93 (2014)(PMID:24550003)

Immunoprecipitation

  1. Maida Y et al. Involvement of telomerase reverse transcriptase in heterochromatin maintenance. Mol Cell Biol. 34, 1576-93 (2014)(PMID:24550003)
  2. Maida Y et al.De Novo RNA Synthesis by RNA-Dependent RNA Polymerase Activity of Telomerase Reverse Transcriptase. Mol Cell Biol. 36, 1248-59 (2016)(PMID:26830230)
  3. Takahashi M et al. Eribulin penetrates brain tumor tissue and prolongs survival of mice harboring intracerebral glioblastoma xenografts. Cancer Sci. 110, 2247-2257 (2019)(PMID:31099446)

Immunocytochemistry

  1. Maida Y et al. Involvement of telomerase reverse transcriptase in heterochromatin maintenance. Mol Cell Biol. 34, 1576-93 (2014)(PMID:24550003)
  • The availability is based on the information in Japan at 17:00, Apr 19, 2024 in JST.
  • The special price is shown in red color.
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  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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